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Plasmid#104583PurposeContains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functionsDepositorInsertHuman IgG1 CH1/CH2/CH3 (IGHG1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM43
Plasmid#133652PurposeIntegration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorInsertp1k-VPS4-DuDre (VPS4 Budding Yeast)
UseTagsDuDreExpressionYeastMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA3790
Plasmid#70110PurposemtDNA elimination/production of rho-0 cellsDepositorInsertsEGFP
hUNG1 Y147A
Neo
UseTagsNone, human OTC mitochondrial targeting sequence,…ExpressionMammalianMutationY147A, aa's 1-77 removedPromoterCMV, EF1a, and SV40 and nativeAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC3.1-caggs-Crimson-CAAX
Plasmid#190041PurposeRed Fluorescent Protein for Long-Term Labeling of Fine Structures in NeuronsDepositorInsertCrimson
UseTagsCAAXExpressionMammalianMutationPromotercaggsAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hPDGFRA-GFP
Plasmid#22919DepositorInserthuman platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-P4M-SidM
Plasmid#51469PurposeBiosensor for PtdIns4PDepositorInsertP4M-SidM(546-647)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCH67 (crRNA expression vector)
Plasmid#217337PurposeCROP-seq crRNA expression vector with GFP and puro markerDepositorInsertAsDR-filler-AsDR8, GFP-puro
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)
Plasmid#128164PurposeUV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSGDlucV3.0
Plasmid#119760Purposedual luciferase with FMDV StopGo sequences flanking MCSDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterSV40 and T7Available sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208 CMV sTurboID (C)-HA-FRB-ERM
Plasmid#153007PurposeExpresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membraneDepositorInsertsTurboID (C) (G74)-ERM (Cb5)
UseLentiviralTagsFRB and HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only