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pET28a-KinN

Plasmid

#139593

Purpose

expresses KinN in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinN

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a D315A

Plasmid

#139592

Purpose

expresses SznF D315A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF D315A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed apartate 315 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF

Plasmid

#139564

Purpose

expresses SznF in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1NhNABP2C

Plasmid

#128312

Purpose

Express chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP1N+hNABP2C (NABP1, NABP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP1N+hNABP2C

Promoter

T7

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbu

Plasmid

#129637

Purpose

Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbu

Use

Tags

His6-tag

Expression

Bacterial

Mutation

V14A and G107H

Promoter

T7

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbuT1A

Plasmid

#129638

Purpose

Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbuT1A

Use

Tags

His6-tag

Expression

Bacterial

Mutation

T1A, V14A and G107H

Promoter

T7

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-FOXP2_P6

Plasmid

#122649

Purpose

Expresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-FOXP2_P1

Plasmid

#122645

Purpose

Expresses truncated variant (aa271-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-FOXP2_P3

Plasmid

#122647

Purpose

Expresses truncated variant (aa367-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-FOXP2_P4

Plasmid

#122648

Purpose

Expresses truncated variant (aa503-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-GBdm

Plasmid

#129690

Purpose

express E. coli GreB E82C/C68S

Depositor

Jeff Gelles

Article

Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GreB E82C C68S (greB E. coli)

Use

Tags

his6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IL- signal_YPet

Plasmid

#128030

Purpose

Mammalian expression vector with N terminal IL-2 signal peptide and YPet

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

IL-2 signal peptide and YPet

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28B-PU.1-ETS-H3

Plasmid

#124435

Purpose

Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis Tag

Expression

Bacterial

Mutation

Residues 225 to 239 replaced with corresponding s…

Promoter

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1-N

Plasmid

#128310

Purpose

Express hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (OB fold, 1–140 aa)

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2-C

Plasmid

#128309

Purpose

Express hNABP2 C-terminus(122–211 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 C-terminal domain (122–211 aa) (NABP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

C-terminal domain (122–211 aa)

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-INTS3-C

Plasmid

#128642

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 C-termini (INTS3 Human)

Use

Tags

His tag on N and C-terminal

Expression

Bacterial

Mutation

INTS3 C-termini (514–1042 aa)

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3/S3

Plasmid

#124627

Purpose

Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 237 to 241 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-MetN

Plasmid

#114458

Purpose

Expression of hexahistidine-tagged AceS methylase in E. coli

Depositor

Patrick Caffrey

Article

Sheehan et al Mol Biosyst. 2017 May 2;13(5):866-873. d

Insert

aceS

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge