-
Plasmid#110848PurposeLentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)DepositorInsertCas9-VQR
UseLentiviralTags3X FLAGExpressionMutationD1135V, R1335Q, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3-P2A-Puro
Plasmid#110838PurposeLentiviral vector for constitutive expression of BE3 (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3-2X
Plasmid#110835PurposeCMV expression vector for BE3-2X construct (not codon optimized)DepositorInsertBE3-2X
UseTagsExpressionMammalianMutation2 tandem NLS sequences in the XTEN linkerPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3-FNLS
Plasmid#110836PurposeCMV expression vector for BE3-FNLS construct (not codon optimized)DepositorInsertBE3-FNLS
UseTags3X FLAGExpressionMammalianMutationNLS sequence at the N-terminusPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRER-P2A-Puro
Plasmid#110849PurposeLentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)DepositorInsertCas9-VRER
UseLentiviralTags3X FLAGExpressionMutationD1135V, G1218R, R1335E, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1-P2A-Puro
Plasmid#110850PurposeLentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)DepositorInsertCas9-HF1
UseLentiviralTags3X FLAGExpressionMutationN497A, R661A, Q695A, Q926APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VQR-PGK-Puro
Plasmid#110855PurposeLentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)DepositorInsertCas9-VQR
UseLentiviralTags3X FLAGExpressionMutationD1135V, R1335Q, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VRER-PGK-Puro
Plasmid#110856PurposeLentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)DepositorInsertCas9-VRER
UseLentiviralTags3X FLAGExpressionMutationD1135V, G1218R, R1335E, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGC-pcoCas9
Plasmid#52256PurposeA binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNADepositorInsert35SPPDK:pcoCas9:NOS cassette
UseCRISPR; Plant expressionTagsExpressionMutationPromoterconstitutive 35SPPDK promoterAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMH1741
Plasmid#160930PurposeExpression of soluble Sc octamer (H3)DepositorInsertsHHF1
HTA1
HTB1
HHT1
UseTags6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…ExpressionMutationcodon-optimized for E. coli -- see provided seque…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMH1742
Plasmid#160929PurposeExpression of soluble Sc octamer (Cse4)DepositorInsertsHHF1
HTA1
HTB1
Cse4
UseTags6His-TEV, N-terminal 6His-TEV, and noneExpressionMutationcodon-optimized for E. coli -- see provided seque…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pML597
Plasmid#32235DepositorInsertmflpe
UseTagsExpressionBacterialMutationM.tuberculosis codon optimized flpe genePromoterAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPGKFLPobpA
Plasmid#13793PurposeExpression of mouse codon-optimized FLP recombinase with bovine growth hormone polyadenylation (bpA) sequence.DepositorInsertFLPo
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPGKPhiC31obpA
Plasmid#13795PurposeExpression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.DepositorInsertPhiC31o
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ABCF2_STOP
Plasmid#221425PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertABCF2 (ABCF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2 _NSP1
Plasmid#169376PurposeCell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFPDepositorInsertSARS-COV-2 _NSP1
UseCell-free expression vectorTagsHis and eGFPExpressionMutationCodon optimized for Saccharomyces cerevisiaePromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)
Plasmid#160647Purposemodule containing the human codon optimized Cas9 and the BASTA selection markerDepositorInsertBASTA / Cas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnos, P35SAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 KIBRA WW domain #1
Plasmid#104229PurposeBacterial expression of WW domain #1 from KIBRADepositorInsertKIBRA WW domain #1 (WWOX Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 KIBRA WW domain #2
Plasmid#104230PurposeBacterial expression of WW domain #2 from KIBRADepositorInsertKIBRA WW domain #2 (WWC1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
MYLK4
Plasmid#39175PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertMYLK4 (MYLK4 Human)
UseBaculovirus expressionTagsHis TevExpressionMutationN371S, codon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only