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Plasmid#169721PurposeMammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)DepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217KPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWKS6
Plasmid#139413PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mMaroon1 marker.DepositorInsertmMaroon1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASt-FMW-attB-AthHen1
Plasmid#104958PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 driversDepositorInsertHen1 (HEN1 Mustard Weed)
UseTagsFLAG MycExpressionInsectMutationPromoterHsp70 promoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human USP7_H456A-3X FLAG
Plasmid#225335PurposeLentiviral expression of human mutant USP7 with 3x FLAG tag in mammalian cellsDepositorInsertUSP7 (USP7 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationH456APromoterEF1aAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp1
Plasmid#218285PurposeAmplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanismDepositorInsertHO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-BSD
Plasmid#216123PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pREMI-ZA
Plasmid#183736PurposePlasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffiiDepositorInsertSh ble
UseRandom insertional gene deletion in komagataella …TagsExpressionMutationPromoterTEF1 promoterAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD313
Plasmid#212931PurposeEncodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSed1 (SED1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPEE42
Plasmid#139421PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.DepositorInsertCitrine
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEE45
Plasmid#139424PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.DepositorInsertmTurquoise1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE44
Plasmid#139423PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.DepositorInsertmTFP1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE43
Plasmid#139422PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.DepositorInsertmMaroon1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS8
Plasmid#139415PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTFP1 marker.DepositorInsertmTFP1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS17
Plasmid#139420PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised mCherry marker.DepositorInsertmCherry
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SNORD2 probe
Plasmid#73070Purposeantisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)DepositorInsertSNORD2 (SNORD2 Human)
UseTagsExpressionMammalianMutationPromotert7Available sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SNORD60 probe
Plasmid#73071Purposeantisense probe for detection of human SNORD60 (C/D box snoRNA U60)DepositorInsertSNORD60 (SNORD60 Human)
UseTagsExpressionMammalianMutationPromotert7Available sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)
Plasmid#115432PurposeVector for expressing RPL28 with mutagenized exon2DepositorInsertsUseTagsExpressionYeastMutationexon 2 deleted, with MfeI / NheI cloning sitePromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSN067
Plasmid#101748PurposeSingle copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-EGFP
Plasmid#84041Purpose3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNTI647 dCas9-Mxi1 TetR KanMX
Plasmid#139474PurposeIntegrates CRISPRi effector and tet-responsive regulatorDepositorInsertsdCas9-Mxi1
Tet Repressor
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1 and pTEF1Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only