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Showing: 1321 - 1340 of 15679 results

  1. HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector

    Plasmid
    #73702
    Purpose
    lentiviral expression of mRIPK3 mutant
    Depositor
    Francis Chan
    Insert
    RIPK3 (Ripk3 Mouse)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    448-AAAA-451
    Promoter
    CMV/tet
    Available since
    March 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. pPyCAG-Nanog(K137A)-IP

    Plasmid
    #62996
    Purpose
    Expresses Mouse Nanog K137A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K137A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Lysine 137 (138 AA in Mouse Protein) to A…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62996-plasmid-map-sequence-id-104598

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  3. pPyCAG-Nanog(Y119A)-IP

    Plasmid
    #62991
    Purpose
    Expresses Mouse Nanog Y119A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y119A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 119 (120 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62991-plasmid-map-sequence-id-104440

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  4. pLVX ALIX-GFP S718/721A

    Plasmid
    #138583
    Purpose
    Expresses fluorescent ALIX S718/721A in mammalian cells
    Depositor
    Wesley Sundquist
    Insert
    ALIX (PDCD6IP Human)
    Use
    Lentiviral
    Tags
    GFP and h30
    Expression
    Mammalian
    Mutation
    changed serines 718 and 721 to alanines
    Promoter
    TREtight
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    138583-plasmid-map-sequence-id-322303

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  5. pLenti puro HA-LMNB1

    Plasmid
    #227591
    Purpose
    Lentiviral plasmid expressing human LMNB1 protein
    Depositor
    Melina Fan
    Insert
    LMNB1 (LMNB1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    227591-plasmid-map-sequence-id-449977

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  6. MEK1-DD-V5

    Plasmid
    #217298
    Purpose
    Lentivirus vector for expression of MEK1 S218D/S222D
    Depositor
    Channing Der
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    S218D/S222D
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    217298-plasmid-map-sequence-id-441105

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  7. MEK1-E102-I103del-V5

    Plasmid
    #217301
    Purpose
    Lentivirus vector for expression of MEK1 E102-I103deletion
    Depositor
    Channing Der
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    E102-I103deletion
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217301-plasmid-map-sequence-id-431010

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  8. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

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  9. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid
    #132622
    Purpose
    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges
    Depositor
    Constance Ciaudo
    Insert
    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    Use
    Lentiviral; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    132622-plasmid-map-sequence-id-259572

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  10. pX459-puro-hCASP8

    Plasmid
    #185378
    Purpose
    For mammalian expression of guide RNA: AAGCAATCTGTCCTTCCTGA that targets human CASP8 (Caspase 8)
    Depositor
    Ayaz Najafov
    Insert
    CASP8 (CASP8 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185378-plasmid-map-sequence-id-363829

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  11. pX459-puro-hARAF-1

    Plasmid
    #185367
    Purpose
    For mammalian expression of guide RNA: caccgTGGTCTACCGACTCATCAAG that targets human ARAF
    Depositor
    Ayaz Najafov
    Insert
    ARAF (ARAF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185367-plasmid-map-sequence-id-363617

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  12. pLKO-puro-mAK1-shRNA-1

    Plasmid
    #185364
    Purpose
    For mammalian expression of shRNA: ATCTTGACTCCCTGAAGTAAC that targets mouse AK1 (adenylate kinase)
    Depositor
    Ayaz Najafov
    Insert
    AK1 (Ak1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185364-plasmid-map-sequence-id-363607

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  13. pMSCV_puro_41589

    Plasmid
    #111632
    Purpose
    Complementation of Dicer_KO mESCs with mutated human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (K70A) (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K70A
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    111632-plasmid-map-sequence-id-217766

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  14. p667-UBC-hcRed-V5_IDG-K

    Plasmid
    #135239
    Purpose
    Gateway destination clone of hcRed (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    hcRed-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135239-plasmid-map-sequence-id-269113

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  15. p663-UBC-V5-eGFP_IDG-K

    Plasmid
    #135265
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135265-plasmid-map-sequence-id-268086

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  16. pHAGE-CMV-Flag-hcRed_IDG-K

    Plasmid
    #135274
    Purpose
    Gateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-hcRed
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    135274-plasmid-map-sequence-id-267146

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  17. pSBbi-Pur-AktAR2-D

    Plasmid
    #125196
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2-D
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125196-plasmid-map-sequence-id-243601

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  18. pPyCAG-Nanog(Q124A)-IP

    Plasmid
    #62993
    Purpose
    Expresses Mouse Nanog Q124A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q124A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Glutamine 124 (125 AA in Mouse Protein) t…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62993-plasmid-map-sequence-id-107891

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  19. pPyCAG-Nanog(M125A)-IP

    Plasmid
    #62994
    Purpose
    Expresses Mouse Nanog M125A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog M125A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Methionine 125 (126 AA in Mouse Protein) …
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62994-plasmid-map-sequence-id-107893

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  20. pPyCAG-Nanog(Q138A)-IP

    Plasmid
    #62997
    Purpose
    Expresses Mouse Nanog Q138A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q138A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Glutamine 138 (139 AA in Mouse Protein) t…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62997-plasmid-map-sequence-id-104599

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Showing: 1321 - 1340 of 15679 results