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Plasmid#113946Purposemammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R2 (TAS1R2 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationresidues 22-839PromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FLAG-METTL3
Plasmid#160250PurposeExpress FLAG-METTL3 in human cellsDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC APEX2-3xFLAG-IRES-eGFP
Plasmid#214983PurposeCytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).DepositorInsertAPEX2 (APX2 Glycine max (soybean))
UseLentiviralTags3xFLAGExpressionMutationPromoterUBCAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_D210N
Plasmid#111904PurposeExpress D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1DepositorInsertRNASEH1 (RNASEH1 Human)
UseTagsV5ExpressionMammalianMutationChanged D 210 to NPromoterAvailable sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
UseTagsExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP3_LAP
Plasmid#101849PurposeTet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCharged Multivesicular Body Protein 3 (CHMP3 Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-m-Flt4
Plasmid#119232PurposePlasmid encodes full-length murine VEGFR-3 gene cloned in mammalian expression vector.DepositorInsertVegfr3 (Flt4 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Raf-1 GST RBD 1-149
Plasmid#13338DepositorInsertRaf-1 (RAF1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 19
Plasmid#134987PurposeExpression of human Myo 19 in mammalian cells.DepositorInsertMyo 19 (MYO19 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP396
Plasmid#149336PurposeBacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobodyDepositorInsertanti-GFP nanobody
UseTagsBiotin acceptor peptide (Avi-tag), His14, and SUM…ExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only