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pDN41

Plasmid

#61344

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-biNLS9 and PKIt NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-blueCKAR(T/A)

Plasmid

#118473

Purpose

Negative-control mutant for blueCKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueCKAR(T/A)

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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p5078 pcDNA4c hBrd4 (1-470)

Plasmid

#14454

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-470) (BRD4 Human)

Use

Tags

Expression

Mammalian

Mutation

amino acids 1-470

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-hBIN1-V5

Plasmid

#27303

Depositor

Robin Shaw

Article

Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

Insert

bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
FH-TET3-CXXCmut-pEF

Plasmid

#127895

Purpose

Expresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAA

Depositor

Patrick Hogan, Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

TET3-CxxCmut (TET3 Human)

Use

Tags

HA and Flag

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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CALID

Plasmid

#169708

Purpose

Cre-activated light-inducible Dre recombinase

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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mH2A2-CT-MYC

Plasmid

#45170

Depositor

Hunt Willard, Brian Chadwick

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy2 (MACROH2A2, H2AFY2 Human)

Use

Tags

HIS and MYC

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Article

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Synthetic Biology; Gateway

Tags

Synthetic Intron and mGFP

Expression

Worm

Mutation

Promoter

Pcol-19

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-blueAKAR(T/A)

Plasmid

#118471

Purpose

Negative-control mutant for blueAKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueAKAR(T/A)

Use

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKCsub-YPet-NES

Plasmid

#138207

Purpose

Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKCsub-YPet-NES

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2021

Availability

Academic Institutions and Nonprofits only

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