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pAAV-hSyn-GRAB_r5-HT1.0

Plasmid

#208719

Purpose

Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Red fluorescent 5-HT sensor GRAB_r5-HT1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-TYR

Plasmid

#32781

Depositor

Ruth Halaban

Article

Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

Insert

Tyrosinase (TYR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pDRM55 tet-AR(1-707)

Plasmid

#183503

Purpose

Lentiviral vector expressing a doxycycline-inducible constitutively active truncated androgen receptor (wild type AR)

Depositor

Wayne Tilley

Article

Hickey et al Nat Med. 2021 Feb;27(2):310-320. doi: 10

Insert

Androgen receptor (AR Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Constitutively active truncated AR; spans AR 1-70…

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

Plasmid

#214996

Purpose

Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM115 (TMEM115 Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

UBC

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Human IFN-gamma promoter/enhancer

Plasmid

#17598

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter with 1st intron enhancer (IFNG Human)

Use

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter plus 680 bp of IFN-gam…

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pINDUCER11 (miR-RUG)

Plasmid

#44363

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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MCP-M-MLV RT∆RNase H

Plasmid

#213749

Purpose

For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

MCP-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCMX-Gal4-TEAD3

Plasmid

#33106

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD3 (TEAD3 Human)

Use

Tags

Gal4

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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