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p206-Switch-ON

Plasmid

#217885

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTALdNC-AAVS1_T1

Plasmid

#80495

Purpose

AAVS1 TALEN expression vector (Left)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 TALEN (Left)

Use

Gateway entry

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTALdNC-AAVS1_T2

Plasmid

#80496

Purpose

AAVS1 TALEN expression vector (Right)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 TALEN (Right)

Use

Gateway entry

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pKM200

Plasmid

#13079

Depositor

Kenan Murphy

Article

Murphy et al BMC Mol Biol. 2003 Dec 13. 4():11.

Insert

Ptac-gam-bet-exo operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR2-V5/HIS

Plasmid

#205617

Purpose

Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

VEGFR2 receptor tyrosine kinase (KDR Human)

Use

Tags

V5-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AnkyrinB-2XHA

Plasmid

#31057

Depositor

Vann Bennett

Article

Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

Insert

Ankyrin B (ANK2 Human)

Use

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pMRBad-Z-CspGFP

Plasmid

#40730

Depositor

Brian McNaughton

Article

Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

Insert

Leucine Zipper prime - C super positive Green Fluorescent Protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBad

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαq*-BERKY3

Plasmid

#158217

Purpose

BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2 (Blast)

Plasmid

#80921

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2

Plasmid

#88917

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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