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Plasmid#135275PurposeGateway destination clone of GFP (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-GFP
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-Nanog(K140A)-IP
Plasmid#62998PurposeExpresses Mouse Nanog K140A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).DepositorInsertMouse Nanog K140A Mutant (Nanog Mouse)
UseTagsExpressionMammalianMutationChanged Lysine 140 (141 AA in Mouse Protein) to A…PromoterAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBCN39-R4R3
Plasmid#34914DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-puro-hARAF-shRNA-1
Plasmid#185370PurposeFor tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAFDepositorInsertARAF (ARAF Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Puro 2.0
Plasmid#24970DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 EGFP pBabe puro
Plasmid#13605DepositorInsertintegrin alpha 9 (ITGA9 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBCN40-R4R3
Plasmid#34915DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lazarus Gata 3 flag
Plasmid#80135Purposeretroviral expression of Gata3-FlagDepositorInsertGATA binding protein 3 (Gata3 Mouse)
UseRetroviralTagsFLAG and IRES-EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Puro_LPAR_AKAR-mut
Plasmid#125207PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporterDepositorInsertLPAR_AKAR-mut
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
1358 pBabe puroL FKHR AAA delta DB
Plasmid#10697DepositorInsertFKHR AAA deltaDB (FOXO1 Human)
UseRetroviralTagsHAExpressionMammalianMutationT24A S256A S319A , deletion in aa 208-220PromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-Nanog(L122A)-IP
Plasmid#62992PurposeExpresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).DepositorInsertMouse Nanog L122A Mutant (Nanog Mouse)
UseTagsExpressionMammalianMutationChanged Leucine 122 (123 AA in Mouse Protein) to …PromoterAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKG212
Plasmid#69758PurposeRetroviral expression of CENP-T aa 1-242DepositorInsertCENP-T aa 1-242 (CENPT Human)
UseRetroviralTagsLacI-NLS and mCherryExpressionMammalianMutatione.coli optimized sequencePromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_puro_41584
Plasmid#111630PurposeComplementation of Dicer_KO mESCs with wild type human DICER isoformDepositorInsertFLAG-hsDicer (DICER1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationWTPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro3+mRFP-Utr_CH
Plasmid#64358Purposebicistronic expression of Utrophin-mRFP and Puro resistanceDepositorInsertUtrophin-mRFP (UTRN Human)
UseTagsIRES-Puro and mRFPExpressionMammalianMutationQ236R; aa1-261 Utrophin presentPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXP CMV/TO RasV12 (w364-1)
Plasmid#22268DepositorInsertHRAS G12V (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationGlycine 12 to Valine (G12V)PromoterAvailable sinceFeb. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pResQ shFEN3 3XF-FEN1 D181A
Plasmid#17753DepositorInsertFlap Endonuclease I (FEN1 Human)
UseLentiviral and RNAiTagsTriple FlagExpressionMammalianMutationD181APromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-dsRed_IDG-K
Plasmid#135262PurposeGateway destination clone of dsRed (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-dsRed
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-kLANA-puro
Plasmid#131699Purposeexpresses the KHSV LANA promoter driving puromycin resistanceDepositorArticleInsertLANA promoter
UseTagsExpressionMammalianMutationPromoterLANAAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-Nanog(Y136A)-IP
Plasmid#62995PurposeExpresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).DepositorInsertMouse Nanog Y136A Mutant (Nanog Mouse)
UseTagsExpressionMammalianMutationChanged Tyrosine 136 (137 AA in Mouse Protein) to…PromoterAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector
Plasmid#73707Purposelentiviral expression of mRIPK3 mutantDepositorInsertRIPK3 (Ripk3 Mouse)
UseLentiviralTagsFlag and HAExpressionMammalianMutationT231A/S232APromoterCMV/tetAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only