Addgene

We narrowed to 4,874 results for: hwa

Showing: 1301 - 1320 of 4874 results

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Paxillin DN

Plasmid

#186151

Purpose

For expressing recombinant paxillin variants in bacteria.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Paxilln residues 1-321 added to N-terminus of WT …

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pJH76

Plasmid

#172144

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.2TAG, luxAB; Plpp pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

gIII(P29*, Y184*)

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-AUR1-msGFP2

Plasmid

#177101

Purpose

Expresses Aur1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

AUR1 (AUR1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-RBD2-6xmGFP

Plasmid

#177109

Purpose

Expresses Rbd2-6xmGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

RBD2 (RBD2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC2-msGFPx3

Plasmid

#177110

Purpose

Expresses Sec2-msGFPx3 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

SEC2 (SEC2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJH578

Plasmid

#172162

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2_v2); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH581

Plasmid

#172165

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2+C); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH767

Plasmid

#172172

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WT]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH857

Plasmid

#172175

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH202

Plasmid

#172150

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

gIII(P29*); 32A pylS variant

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH223

Plasmid

#172151

Purpose

pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/lac (pylBCD 34B_sub-pop5); LacI

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E178K, E251D) PylD(A252V)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH224

Plasmid

#172152

Purpose

pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/lac (pylBCD 40C_sub-pop6); LacI

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH463

Plasmid

#172155

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 36A_sub-pop1); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E122K, E178K, G188R)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH573

Plasmid

#172157

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (Untagged pylBCD); AraC

Use

Tags

Expression

Bacterial

Mutation

Codon-optimized Methanosarcina acetivorans pylBCD

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH574

Plasmid

#172158

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 36A_sub-pop2); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K) PylC(E316D)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH575

Plasmid

#172159

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop3); AraC

Use

Tags

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH576

Plasmid

#172160

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop4); AraC

Use

Tags

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K, E251D) PylD(A252V)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH19

Plasmid

#172141

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

gIII(P29*)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH60

Plasmid

#172142

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.CD

Use

Tags

Expression

Bacterial

Mutation

PylCD partial operon (PylB deleted to assess impa…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH77

Plasmid

#172145

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.3TAG, luxAB; Plpp pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

gIII(P29*, P83*, Y184*)

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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