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pCFJ1320
Plasmid#84829PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRY-Gal∆DD (B1013)
Plasmid#92035PurposeExpresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRESDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationFusion of plant CRY2 to Gal4 binding domain (AA1-…PromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Venus-Puma-pEGFP-C1
Plasmid#166739PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, PumaDepositorInsertPuma (BBC3 Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
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CRY-GalVP16l (B694)
Plasmid#92030Purposeexpresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGalBD-VP16ExpressionMammalianMutationNLS sequence of CRY2 mutatedPromoterCMVAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO2-RC_B
Plasmid#145981PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
UseTagsExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGateway 3XFlag Prickle2 flag tagged
Plasmid#24645DepositorInsertPrickle 2 (PRICKLE2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceApril 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmCherry Tensin2
Plasmid#105300PurposeTo study localization and function of Tensin 2 with EGFP Tensin1DepositorInsertTensin2 (TNS2 Human)
UseTagsmCherryExpressionMammalianMutationlacks N-terminal C1 domainPromoterAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot2
Plasmid#79255PurposeExpresses EGFP-tagged DmNot2 in S2 cellsDepositorInsertDmNot2 (Rga Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCCr4
Plasmid#79252PurposeExpresses EGFP-tagged DmCCr4 in S2 cellsDepositorInsertDmCaf40 (twin Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-S (P.1 lineage / Brazilian variant)
Plasmid#169847PurposeMammalian expression plasmid for S of SARS-CoV-2 (P.1 lineage / Brazilian variant) with N-terminal c-myc tagDepositorInsertProtein S
UseTagsHA leader and c-myc tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-S (B.1.1.7 lineage / UK variant)
Plasmid#169848PurposeMammalian expression plasmid for S of SARS-CoV-2 (B.1.1.7 lineage / UK variant) with N-terminal c-myc tagDepositorInsertProtein S
UseTagsHA leader and c-myc tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)
Plasmid#194233PurposeMammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domainDepositorInsertCD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-wdb
Plasmid#195069PurposeExpresses FLAG-tagged Drosophila wdb proteinDepositorInsertwiderborst (wdb Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1B-EGFP-DmSquash_B
Plasmid#145926PurposeInsect Expression of DmSquashDepositorInsertDmSquash (squ Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
N1-mSin1.2-GFP
Plasmid#72908PurposeGFP-tagged mSin1 (isoform 2)DepositorInsertTarget of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only
