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Shank1 scFv [N22/21]

Plasmid

#190539

Purpose

Mammalian Expression of Shank1 scFV. Derived from hybridoma N22/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Shank1 (Rattus norvegicus) recombinant scFV (Shank1 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Pan-Shank scFv [N23B/49]

Plasmid

#190542

Purpose

Mammalian Expression of Pan-Shank scFV. Derived from hybridoma N23B/49.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Shank (Rattus norvegicus) recombinant scFV (Shank2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Shank2 scFv [N23B/6]

Plasmid

#190543

Purpose

Mammalian Expression of Shank2 scFV. Derived from hybridoma N23B/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Shank2 (Rattus norvegicus) recombinant scFV (Shank2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Rufy3 scFv [N483/126]

Plasmid

#190557

Purpose

Mammalian Expression of Rufy3 scFV. Derived from hybridoma N483/126.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Rufy3 (Mus musculus) recombinant scFV (Rufy3 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Rufy3 scFv [N483/84]

Plasmid

#190559

Purpose

Mammalian Expression of Rufy3 scFV. Derived from hybridoma N483/84.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Rufy3 (Mus musculus) recombinant scFV (Rufy3 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Rufy3 scFv [N483/126]

Plasmid

#182089

Purpose

Mammalian Expression of Rufy3 scFv. Derived from hybridoma N483/126.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Rufy3 (Mus musculus) (Rufy3 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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RGS14 scFv [N133/21]

Plasmid

#182081

Purpose

Mammalian Expression of RGS14 scFv. Derived from hybridoma N133/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting RGS14 (Rattus norvegicus) (Rgs14 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/81]

Plasmid

#182073

Purpose

Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-zGrad

Plasmid

#119716

Purpose

Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

Depositor

Holger Knaut

Article

Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

Insert

zGrad

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pComb3HSS

Plasmid

#64133

Purpose

2nd generation plasmid for phage display on modified geneIII, contains stuffer fragment

Depositor

Carlos Barbas

Article

Barbas et al Methods, A companion to Methods in Enzym

Inserts

SS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer

Use

Phage display

Tags

geneIII

Expression

Bacterial

Mutation

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSLC-284

Plasmid

#124294

Purpose

A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

Depositor

Swaine Chen

Article

Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge

Insert

vsfGFP-9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCrl

Plasmid

#122443

Purpose

Expresses entire mouse reelin ORF in mammalian cells

Depositor

Tom Curran

Article

D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

Insert

reelin (Reln Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pUltra-FPheKRS

Plasmid

#136631

Purpose

Genetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteins

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

FPheKRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Tac1 promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mouse-DJ1-HA

Plasmid

#29396

Depositor

Mark Cookson

Article

Miller et al Neurosci Lett. 2005 Feb 21. 374(3):203-6

Insert

DJ1 (Park7 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Plasmid

#60907

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINVL

Plasmid

#26040

Depositor

Ray Owens

Article

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ

Use

Tags

u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pOPINVH

Plasmid

#26041

Depositor

Ray Owens

Article

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ-KHHHHHH

Use

Tags

KHHHHHH and u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCrlM

Plasmid

#122444

Purpose

Expresses myc tagged mouse reelin ORF in mammalian cells

Depositor

Tom Curran

Article

D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

Insert

reelin (Reln Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Tras light chain

Plasmid

#216294

Purpose

Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Tras light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Pert Light chain

Plasmid

#216310

Purpose

Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Pert Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge