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Addgene
Showing: 1301 - 1320 of 1572 results

  2. Gi1-CASE

    Plasmid
    #168120
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A121/E122 within GNAI1
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    168120-plasmid-map-sequence-id-332858

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  3. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    168122-plasmid-map-sequence-id-332862

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  4. G15-CASE

    Plasmid
    #168126
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at E244/N245 within GNA15
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    168126-plasmid-map-sequence-id-332871

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  5. pcDNA3-C16orf59-mVen

    Plasmid
    #211425
    Purpose
    mammalian expression of human C16orf59 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C16orf59 (C16orf59 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211425-plasmid-map-sequence-id-419247

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  6. px330_PTEN exon 5 gRNA

    Plasmid
    #68348
    Purpose
    px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    PTEN exon 5 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68348-plasmid-map-sequence-id-119620

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  7. px330_P53 exon 8 gRNA

    Plasmid
    #68349
    Purpose
    px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 8 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68349-plasmid-map-sequence-id-119621

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  8. px330_SMAD exon 2 gRNA

    Plasmid
    #68350
    Purpose
    px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 2 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68350-plasmid-map-sequence-id-119622

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  9. px330_P53 exon 7 gRNA

    Plasmid
    #68351
    Purpose
    px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 7 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68351-plasmid-map-sequence-id-119623

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  10. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 9 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68352-plasmid-map-sequence-id-119624

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  11. pcDNA3-Cdc45-mVen

    Plasmid
    #211390
    Purpose
    mammalian expression of human Cdc45 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    Cdc45 (CDC45 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211390-plasmid-map-sequence-id-420427

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  12. pcDNA3-Mcm5-mVen

    Plasmid
    #211394
    Purpose
    mammalian expression of human Mcm5 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    Mcm5 (MCM5 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211394-plasmid-map-sequence-id-419259

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  13. pcDNA3-C17orf53-mVen

    Plasmid
    #211421
    Purpose
    mammalian expression of human C17orf53 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211421-plasmid-map-sequence-id-419256

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  16. pcDNA3-Mcm2-mVen

    Plasmid
    #211398
    Purpose
    mammalian expression of human Mcm2 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    Mcm2 (MCM2 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211398-plasmid-map-sequence-id-419249

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  17. pcDNA3-C17orf53_1-87-mVen

    Plasmid
    #211442
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211442-plasmid-map-sequence-id-419261

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  19. pcDNA3-C17orf53_1-241-mVen

    Plasmid
    #211444
    Purpose
    mammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Deletion of aa 242-646
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211444-plasmid-map-sequence-id-419266

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Showing: 1301 - 1320 of 1572 results