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Addgene
Showing: 1301 - 1320 of 4565 results
  1. pTSTEP

    Plasmid
    #72334
    Purpose
    Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)
    Depositor
    Insert
    RRS-TagRFPT-p10-[Golic+] RRS-EGFP
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pDsRed-TSTEP_v2

    Plasmid
    #72336
    Purpose
    Tissue-specifically swappable (C-terminal TagRFPT-to-EGFP) genome tagging plasmid with dsRed selection marker for injection mediated gene targeting (Drosophila melanogaster)
    Depositor
    Insert
    RRS-TagRFPT-p10-[3xP3dsRed]-RRS-EGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pMT-HA-STX1

    Plasmid
    #89310
    Purpose
    Expresses HA tagged syntaxin 1 in Drosophila cells
    Depositor
    Insert
    STX1 (Syx1A Fly)
    Use
    Tags
    HA tag
    Expression
    Insect
    Mutation
    Promoter
    P-MT (Metallothionein)
    Availability
    Academic Institutions and Nonprofits only
  4. E2F1 in pGEX4t

    Plasmid
    #112976
    Purpose
    For protein expression and purification of Drosophila E2F1
    Depositor
    Insert
    E2F1 (E2f1 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. CvcE in pGEX4t

    Plasmid
    #112977
    Purpose
    For protein expression and purification of Drosophila CvcE
    Depositor
    Insert
    cycE (CycE Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. K560GFP

    Plasmid
    #129761
    Purpose
    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays
    Depositor
    Insert
    KHC (Khc Fly)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. KRigor980

    Plasmid
    #129763
    Purpose
    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces
    Depositor
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    R210A
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. K406

    Plasmid
    #129764
    Purpose
    Expresses Drosophila Kinesin-1 truncated after neck-coil at 406
    Depositor
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 406
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. K560_AviN_coBirA

    Plasmid
    #129766
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
    Depositor
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1301 - 1320 of 4565 results