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pSynapsin_NLS_GFP_P2A_SNAP_NR1

Plasmid

#170458

Purpose

Expresses SNAP tagged NR1 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

NR1 (Grin1 Rat)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCK069 desmaMCS:minprom_mApple,exorh:EGFP

Plasmid

#195982

Purpose

Expression construct

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EphrinB3-Flag(A227Y)

Plasmid

#155013

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

EphrinB3 (EPHB3 Human)

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

A227Y

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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791 p3E-2A-pCS2MCSpA v2

Plasmid

#195973

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann, Kristen Kwan

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-multiple cloning site

Use

Other

Tags

Expression

Mutation

Promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

Plasmid

#153071

Purpose

Lentiviral vector expressing human ZC3H12D mutated (D95N)

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

Mutated ZC3H12D (D95N) (ZC3H12D Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed D95 to N, known to abrogate the RNase enz…

Promoter

EF1α

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXD13-IDR-mCherry

Plasmid

#145280

Purpose

Bacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13-IDR (HOXD13 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-RUNX2(WT)-IDR-mCherry

Plasmid

#145286

Purpose

Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(WT)-IDR (RUNX2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13-IDR-mCherry-CRY2

Plasmid

#145258

Purpose

Mammalian Expression of Opto HOXD13 IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpe

Plasmid

#79076

Purpose

Expression of mouse Cebpe

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp epsilon (Cebpe Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pBactin-Nedd1-mNeonGreen

Plasmid

#196864

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mNeonGreen (Nedd1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCFP-LacI-HOXD13wt-IDR

Plasmid

#145255

Purpose

Expresses fusion of ECFP-LacI and HOXD13wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBactin-Tubg1-mNeonGreen

Plasmid

#196869

Purpose

Expression of the gamma-tubulin fused to mNeonGreen

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Tubg1-mNeonGreen (Tubg1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-RUNX2-IDR-mCherry-CRY2-NLS

Plasmid

#145267

Purpose

Mammalian Expression of Nuclear Opto RUNX2 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2wt-IDR (RUNX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCK036 desmaMCS:minCerulean,cryaa:Venus

Plasmid

#195981

Purpose

Expression construct

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, cryaa Venus (desma Zebrafish)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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1M-Qt-antiGFP

Plasmid

#206951

Purpose

Mammalian expression of 1M-Qt EMcapsulins targeting GFP

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc, antiGFP-intrabody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCK072 p5E 147bp exorh:EGFP

Plasmid

#195950

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh minimal regulatory elements and EGFP

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-mNeonGreen-CI

Plasmid

#196850

Purpose

Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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RCASBP(A)-3xFlag-mHoxd13+7Ala

Plasmid

#145289

Purpose

Avian Expression of murine SPDH (+7Ala) Hoxd13 with N-terminal 3xFlag tag

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

Hoxd13(+7Ala) (Hoxd13 Mouse)

Use

Viral avian expression

Tags

3xFlag

Expression

Mutation

A-repeat expanded

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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RCASBP(A)-3xFlag-mHoxd13wt

Plasmid

#145288

Purpose

Avian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag tag

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

Hoxd13(wt) (Hoxd13 Mouse)

Use

Viral avian expression

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 C1(259-443)

Plasmid

#89832

Purpose

Express GATA3 C1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

C1 (AA 259-443)

Promoter

CMV

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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