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Plasmid#220279Purposeintegrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombinationDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only
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pEC2796 (pSpy0C4)
Plasmid#220280Purposeintegrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombinationDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP580
Plasmid#222959PurposeExpresses R141S K142S LoaP double mutantDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 141 to Serine and Lysine 142 to …PromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP579
Plasmid#222958PurposeExpresses R36S K51S LoaP double mutantDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 36 to Serine and Lysine 51 to Se…PromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_VPS35L(A830T)
Plasmid#209693PurposeExpresses MAC3-tagged VPS35L(A830T) in mammalian cellsDepositorInsertVPS35L (VPS35L Human)
UseTagsMAC3-tagExpressionMammalianMutationA830T, changed Alanine 830 to ThreoninePromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_VPS35L
Plasmid#209692PurposeExpresses MAC-tagged VPS35L in mammalian cellsDepositorInsertVPS35L (VPS35L Human)
UseTagsMAC-tagExpressionMammalianMutationNC_000016.9:g.19710824G>APromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rif1-Halo HRD
Plasmid#207081PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF168-Halo HRD
Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
UseTagsExpressionMammalianMutationPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-MDC1 PST deletion
Plasmid#207109PurposepHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the PST repeat region in human cells.DepositorInsertHaloTag-MDC1 PST deletion
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF169-Halo HRD
Plasmid#207083PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.DepositorInsertHaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL661-WT(TTTT)
Plasmid#215864PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL661 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-WT(TTTT)
Plasmid#215847PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL864R-WT(TTTT)
Plasmid#215860PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL864R with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-ATTT
Plasmid#215848PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with ATTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL714-WT(TTTT)
Plasmid#215866PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL714 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-CTTT
Plasmid#215852PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with CTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTTA
Plasmid#215851PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTTA stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL864-WT(TTTT)
Plasmid#215858PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL864 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL554-WT(TTTT)
Plasmid#215862PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL554 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTAT
Plasmid#215850PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTAT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TATT
Plasmid#215849PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TATT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-EGFP12-WT(TTTT)
Plasmid#215856PurposeThis plasmid encodes sgRNA that target EGFP with stretch sequenceDepositorInsertsgRNA-EGFP12 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTTC
Plasmid#215855PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTTC stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TCTT
Plasmid#215853PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TCTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTCT
Plasmid#215854PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTCT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-MiniR
Plasmid#208328PurposeMiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.DepositorInsertH2B-MiniR
UseTagsMiniRExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIDAF
Plasmid#213712PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIBY55
Plasmid#213711PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.DepositorInsertEGFP
UseTagsTwin-strep-tag and V5-tagExpressionMammalianMutationPromoterSV40Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISRor9_reRNA
Plasmid#205352PurposeBacterial expression of ISRor9 TnpB and its reRNADepositorInsertISRor9 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISNsp3_reRNA
Plasmid#205351PurposeBacterial expression of ISNsp3 TnpB and its reRNADepositorInsertISNsp3 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISCpe2_reRNA
Plasmid#205335PurposeBacterial expression of ISCpe2 TnpB and its reRNADepositorInsertISCpe2 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISCbt1_reRNA
Plasmid#205330PurposeBacterial expression of ISCbt1 TnpB and its reRNADepositorInsertISCbt1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-PuRRRE
Plasmid#208327PurposeC-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-PuRRRE
UseTagsPuRRREExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-TREER
Plasmid#208325PurposeC-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-TREER
UseTagsTREERExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-TinyERRE
Plasmid#208324PurposeC-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-TinyERRE
UseTagsTinyERREExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
TREER-Actin
Plasmid#208330PurposeTREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.DepositorInsertTREER-Actin
UseTagsTREERExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TOMM20-PuRRRE
Plasmid#208329PurposePuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.DepositorInsertTOMM20-PuRRRE
UseTagsPuRRREExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA TfR1-PunyEEER
Plasmid#208326PurposeC-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).DepositorInsertTfR1-PunyEEER
UseTagsPunyEEERExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISAam1_reRNA
Plasmid#205322PurposeBacterial expression of ISAam1 TnpB and its reRNADepositorInsertISAam1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISAeme8_reRNA
Plasmid#205324PurposeBacterial expression of ISAeme8 TnpB and its reRNADepositorInsertISAeme8 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISAepa1_reRNA
Plasmid#205325PurposeBacterial expression of ISAepa1 TnpB and its reRNADepositorInsertISAepa1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISBce3_reRNA
Plasmid#205327PurposeBacterial expression of ISBce3 TnpB and its reRNADepositorInsertISBce3 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISBth16_reRNA
Plasmid#205328PurposeBacterial expression of ISBth16 TnpB and its reRNADepositorInsertISBth16 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISCath1_reRNA
Plasmid#205329PurposeBacterial expression of ISCath1 TnpB and its reRNADepositorInsertISCath1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISCbt2_reRNA
Plasmid#205331PurposeBacterial expression of ISCbt2 TnpB and its reRNADepositorInsertISCbt2 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISClte2_reRNA
Plasmid#205334PurposeBacterial expression of ISClte2 TnpB and its reRNADepositorInsertISClte2 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISEc44_reRNA
Plasmid#205342PurposeBacterial expression of ISEc44 TnpB and its reRNADepositorInsertISEc44 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISCvi1_reRNA
Plasmid#205336PurposeBacterial expression of ISCvi1 TnpB and its reRNADepositorInsertISCvi1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISCysp13_reRNA
Plasmid#205338PurposeBacterial expression of ISCysp13 TnpB and its reRNADepositorInsertISCysp13 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISCytsp1_reRNA
Plasmid#205339PurposeBacterial expression of ISCytsp1 TnpB and its reRNADepositorInsertISCytsp1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only