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pCDNA3_cAMPFIRE-M

Plasmid

#182280

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCM189-ParB1::mCherry

Plasmid

#87253

Purpose

Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB1

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

pBAD/tetO

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGD-P1/HC-Pro

Plasmid

#196328

Purpose

35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

Depositor

Zhenghe Li

Article

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tobacco etch virus P1/HC-Pro

Use

Tags

Expression

Plant

Mutation

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/38]

Plasmid

#182095

Purpose

Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GFP

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSC101-AmpR EV

Plasmid

#208184

Purpose

empty plasmid vector, non-fluorescent

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Type

Empty backbone

Use

Reporter

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-FoxO1-Clover

Plasmid

#67759

Purpose

Fluorescent fusion protein for tracking Akt signaling

Depositor

Peter Rotwein

Article

Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

Insert

Fox01 (Foxo1 Mouse)

Use

Lentiviral

Tags

Clover

Expression

Mutation

Changed Serine 209 to Alanine, changed Histidine …

Promoter

EF1a

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-HA-OTUB1

Plasmid

#22551

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUB1 (OTUB1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB3

Plasmid

#120288

Purpose

Produce human recombinant TGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)

Use

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-6XHis-FLKSRP

Plasmid

#23001

Depositor

Douglas Black

Article

Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

Insert

KSRP (KHSRP Human)

Use

Tags

EGFP and His

Expression

Mammalian

Mutation

valine inserted at aa#2

Promoter

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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lentiTet-P2A-mCherry BlastR

Plasmid

#186743

Purpose

Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

P2A-mcherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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