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Plasmid#72668PurposePeriplasmic expression of APETx2 in E.coliDepositorInsertAPETx2
UseTagsHis tag, MBP, MalE signal sequence for periplasmi…ExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Twitch-1 pcDNA3
Plasmid#49812PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-1
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-STRAD alpha
Plasmid#14889DepositorInsertSTRAD alpha (STRADA Human)
UseTags6xHis/XpressExpressionMammalianMutationPromoterAvailable sinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)
Plasmid#136646PurposeExpression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1R312A (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJB8
Plasmid#19531DepositorInsertDnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-AKAR4
Plasmid#64733PurposeEndoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.DepositorInsertAKAR4
UseTagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLEx-TetOn3G-3xHA-FLEx
Plasmid#133729PurposePlasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.DepositorInsertTetOn3G-3xHA (floxed & inverted)
UseAAVTags3xHA-tagExpressionMammalianMutationPromoterpCMVAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/V5 hsa-mir-205
Plasmid#26312DepositorInserthsa-mir-205 (MIR205 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBADmod1-linker2-gamS
Plasmid#45833DepositorInsertgamS
UseSynthetic BiologyTagsHistagExpressionBacterialMutationPromoterpBADAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only