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Plasmid#180026PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).DepositorInsertGB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS184CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, T805C, C813S)-Strep
Plasmid#169749PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Threonine 805 to Cysteine…PromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, G808C, C813S)-Strep
Plasmid#169750PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Glycine 808 to Cysteine, …PromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, S712C, P801G, C813S)-Strep
Plasmid#164445PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationSerine 712 to Cysteine, Proline 801 to Glycine, C…PromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, A756C, P801G, C813S)-Strep
Plasmid#164446PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationAlanine 756 to Cysteine, Proline 801 to Glycine, …PromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, C813S, S863C)-Strep
Plasmid#164447PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Cysteine 813 to Serine, S…PromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKG96
Plasmid#63072PurposeRetroviral expression of EGFP-TEV-S-tag CENP-A for protein localization and affinity purification (LAP)DepositorInsertCENP-A (CENPA Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationA12V, C75Y, and L121F mutations compared to GenBa…PromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIC144
Plasmid#63067PurposeRetroviral expression of EGFP-TEV-S-tag CENP-K for protein localization and affinity purification (LAP)DepositorInsertCENP-K, IMAGE clone 3511065 (CENPK Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAP2
Plasmid#171208Purposebacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV proteaseDepositorInsertpgl-3 (pgl-3 Nematode)
UseTagsMBP::HIS::TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKG136
Plasmid#63075PurposeRetroviral expression of EGFP-TEV-S-tag CENP-W for protein localization and affinity purification (LAP)DepositorInsertCENP-W (CENPW Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationE. coli codon optimized, hardened against Dharmac…PromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET29a-human histone 4-in-One
Plasmid#223237PurposePolycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4DepositorInsertsHis6-VSVG-Histone H2A
Histone H2B
Histone H3
Histone H4-TEV-His6-Dual StrepII
UseTagsDual StrepII, His6, TEV protease site, and VSVGExpressionBacterialMutationA103GPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS-mCherry
Plasmid#135457PurposeMammalian mCherry reporter to read out FLARE and SPARK activationDepositorInsertmCherry
UseTagsmCherryExpressionMammalianMutationPromoterUASAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-hKdm2b-CxxC-PHD
Plasmid#49216PurposeBacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.DepositorInsertKdm2b (KDM2B Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJW1666
Plasmid#112040PurposeCEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URADepositorInsertNone
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1505
Plasmid#62359PurposeRPL16a HA-TEV-AVI pop-in/pop-out plasmidDepositorInsertRPL16A
UseYeast taggingTagsHA-TEV-AVIExpressionMutationPromoterAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAP974-1
Plasmid#99497PurposeTEV::linker::tagRFP::3XFlagDepositorInsertTEV::linker::tagRFP::3XFlag
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKG113
Plasmid#63073PurposeRetroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)DepositorInsertCENP-T (CENPT Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationE. coli codon optimized. G217R mutation compared …PromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
ataxin3:1-431_Q79_BV
Plasmid#209733PurposeBacterial expression for structure determinationDepositorInsertAtaxin 3 (ATXN3 Human)
UseTags6xHis-SBP-TEVExpressionBacterialMutationPromoterPolyhedrinAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAP997-1
Plasmid#99500Purpose3XFlag::tagRFP::linker::TEVDepositorInsert3XFlag::tagRFP::linker::TEV
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJW1667
Plasmid#112041PurposeCEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URADepositorInsertERG5
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only