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pCA-G-intron(Neo)-T(LNL)

Plasmid

#36907

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Neo

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 pBS

Plasmid

#13584

Depositor

Dean Sheppard

Article

Yokosaki et al J Biol Chem. 1994 Oct 28. 269(43):26691-

Insert

integrin alpha 9 (ITGA9 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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Gucy1b2-D-IRES-tauGFP-pA-ACNF TV

Plasmid

#105208

Purpose

targeting vector to generate a Gucy1b2-D-IRES-tauGFP mouse strain.

Depositor

Peter Mombaerts

Article

Bleymehl et al Neuron. 2016 Dec 21;92(6):1196-1203. doi

Insert

Gucy1b2-D-IRES-tauGFP-pA-ACNF (Gucy1b2 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only
pBS-AMV-γ1L9'S-nAchR

Plasmid

#20686

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrng Mouse)

Use

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Promoter

Available since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pBS-tRNA-Gly

Plasmid

#174317

Purpose

For in vitro transcription of mouse tRNAs

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

tRNA-gly

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHA850 - osm-10p::FynY531F

Plasmid

#139207

Purpose

Express activated Fyn kinase in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

Y531F

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA852 - mec-4p::FynY531F

Plasmid

#139209

Purpose

Expresses activated Fyn kinase in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

Y531F

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Sph ELL2

Plasmid

#127270

Purpose

For in vitro translation of human WT ELL2 with internal HA tag

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

WT Mets and HA tag at SphI site (8th Met)

Promoter

T7

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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ELL2mRNA+polyA

Plasmid

#127256

Purpose

For in vitro translation of human ELL2 with 30 nt pA tail

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

shortens 3'UTR to 40 bp adds A30 tail

Promoter

T7

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Xba ELL2

Plasmid

#127268

Purpose

For in vitro translation of WT ELL2 with HA tag inserted near N-term

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

Promoter

T7

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only
rat brain inositol 1,4,5-trisphosphate 3-kinase A

Plasmid

#122155

Purpose

rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)

Depositor

Christophe Erneux

Article

Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Insert

inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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FDXR KO-1

Plasmid

#16613

Depositor

Bert Vogelstein

Article

Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

Insert

FDXR knockout (FDXR Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
Comp AlphaPS3

Plasmid

#14071

Depositor

Richard Hynes

Article

Stark et al Development. 1997 Nov . 124(22):4583-94.

Insert

Drosphila Comp Alpha PS3 Integrin (scb Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
integrin beta6 777T pBS

Plasmid

#13581

Depositor

Dean Sheppard

Article

Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

Insert

integrin beta 6 777T (ITGB6 Human)

Use

Tags

Expression

Bacterial

Mutation

777T. Lacks last 11 amino acids of cytoplasmic do…

Promoter

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pInt-ERCreER

Plasmid

#190040

Purpose

This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

Depositor

Kayoko Tanaka

Article

Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

Insert

ERT2-Cre-ERT2 flanked by AAVS1

Use

CRISPR and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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PG13-luc (wt p53 binding sites)

Plasmid

#16442

Purpose

Luciferase reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-GAPDH-IRES-EGFP

Plasmid

#115918

Purpose

donor to insert IRES-EGFP at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-EGFP

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDiCre-mCherry

Plasmid

#164572

Purpose

Integration of a DiCre cassette in PbGFP parasite genome

Depositor

Olivier Silvie

Article

Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d

Inserts

FKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_iPE-N

Plasmid

#214734

Purpose

encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter

Depositor

Gil Westmeyer

Article

Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

Insert

CAG_iPE-N_bGH

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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