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pET28a-ammB3

Plasmid

#167815

Purpose

Expresses His6-tagged AmmB3 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

AmmB3

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ammC1

Plasmid

#167816

Purpose

Expresses His6-tagged AmmC1 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

AmmC1

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-P(11)4

Plasmid

#185786

Purpose

Encodes enhanced green fluorescent protein linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

eGFP-P(11)4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ATA-P(11)4

Plasmid

#185787

Purpose

Encodes enzyme ω-aminotransferase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

ATA-P(11)4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28c_S(-31)-M30_dT7term

Plasmid

#112253

Purpose

Expresses the S(-31)-M30 apta-FRET RNA origami structure.

Depositor

Ebbe Sloth Andersen

Article

Jepsen et al Nat Commun. 2018 Jan 2;9(1):18. doi: 10.

Insert

S(-31)-M30 apta-FRET RNA origami with T7 promoter and terminators.

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28c_S*5-M5_dT7term

Plasmid

#112254

Purpose

Expresses the S*5-M5 apta-FRET RNA origami structure.

Depositor

Ebbe Sloth Andersen

Article

Jepsen et al Nat Commun. 2018 Jan 2;9(1):18. doi: 10.

Insert

S*5-M5 apta-FRET RNA origami with T7 promoter and terminators.

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-P(11)4

Plasmid

#185789

Purpose

Encodes glutathione S-transferase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

GST-P(11)4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b_SNAP-ecDHFR

Plasmid

#187108

Purpose

Expression of SNAP-ecDHFR fusion in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

SNAP-ecDHFR

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b_SNAP-ecDHFRmt

Plasmid

#187109

Purpose

Expression of SNAP-ecDHFRmt fusion in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

SNAP-ecDHFRmt

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

ecDHFR-R44L-H45Q

Promoter

T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET3a-FruK

Plasmid

#186256

Purpose

Overexpression of FruK for purification

Depositor

Emile Van Schaftingen, Liskin Swint-Kruse

Article

Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

Insert

1-phosphofructokinase (fruK E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2343_Tier3(SB)-YPet_Blast

Plasmid

#169641

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven YPet and BlastR expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

PRPBSA

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET24TEV-tSHIP1

Plasmid

#183770

Purpose

Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP1 (INPP5D Human)

Use

Tags

6HIS and Tobacco Etch Virus (TEV) Cleavage site

Expression

Bacterial

Mutation

includes aa1 to aa883 only

Promoter

T7 lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET24TEV-tSHIP2

Plasmid

#183771

Purpose

Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP2 (INPPL1 Human)

Use

Tags

6HIS and Tobacco Etch Virus (TEV) Cleavage site

Expression

Bacterial

Mutation

includes aa18 to aa985 only

Promoter

T7 lac

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET17b-yebST

Plasmid

#175804

Purpose

Complementation of yebST knockout in E. coli K-12

Depositor

Ian Henderson, Gira Bhabha, Damian Ekiert

Article

Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

Insert

yebST

Use

Tags

None

Expression

Bacterial

Mutation

silent mutations to introduce restriction sites

Promoter

T7

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET11-PC(75-228)-H6

Plasmid

#183779

Purpose

Express PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (Pc Fly)

Use

Tags

6 His Tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET11-PC(75-390)-H6

Plasmid

#183781

Purpose

Express PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assays

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (w Fly)

Use

Tags

6 His tag at the C-terminus

Expression

Bacterial

Mutation

6 His tag at the C-terminus

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_PDCD6IP_ALIX V-domain

Plasmid

#178483

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PDCD6IP_ALIX V-domain (PDCD6IP Synthetic, Human)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_IRF3_SMAD/FHA

Plasmid

#178479

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

IRF3_IRF3 (IRF3 Synthetic, Human)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET-epELM-5

Plasmid

#179391

Purpose

Express a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteria

Depositor

Shiro Iuchi

Article

Iuchi et al Commun Biol. 2021 Dec 15;4(1):1399. doi:

Insert

ELMSAN1 (MIDEAS Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

T7 lac

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn2delBRCT

Plasmid

#173939

Purpose

Bacterial expression of PARP1 lacking Zn2 and BRCT domains

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn2delBRCT (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 97-213 and 374-520; V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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