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Plasmid#223889Purpose4G-cloning donor for C-terminal 3C-Venus-His8 tagDepositorInsert3C-Venus-His8
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_CMVC
Plasmid#223862Purpose4G-cloning donor for mammalian promoter and terminator, C-terminal tag addedDepositorInsertCMV promoter and bGH polyA
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH502
Plasmid#215781PurposeNBU2 backbone with LacI(YQR) and P(O1)_sgRNA targeting NanolucDepositorInsertsLacI(YQR)
sgRNA-ribozyme cassette
Nanoluc
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBt1311, P(O1), and cfxAAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-Empty-cleavableELP
Plasmid#219401PurposeInsert protease recognition site with BsaI to make custom protease-responsive elastin-like polypeptide (ELP)DepositorInsertEmpty-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-FXa-cleavableELP
Plasmid#219403PurposeFactor Xa-responsive elastin-like polypeptide (ELP)DepositorInsertFXa-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EK-cleavableELP
Plasmid#219404PurposeEnterokinase-responsive elastin-like polypeptide (ELP)DepositorInsertEK-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-Thr-cleavableELP
Plasmid#219405PurposeThrombin-responsive elastin-like polypeptide (ELP)DepositorInsertThr-cleavableELP
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1051
Plasmid#209541PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertRecombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK1059
Plasmid#209549PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertDownstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ131C-RZ-Lb
Plasmid#134347PurposeGolden Gate entry vector; empty vector to clone the 1st LbCas12a crRNA under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL12022
Plasmid#117510PurposeAtAG promoter Golden Gate Level 0DepositorInsertBsaI:GGAG:pAG:AATG:BsaI
UseTagsExpressionPlantMutationPromoterAtAGAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 'rncS
Plasmid#117129Purposesmc gene upstream homology regionDepositorInsert'rncS
UseGolden gate donor vector for gene targeting in ba…TagsExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGE003
Plasmid#48854PurposeProvides the At1g04880 terminator as GreenGate module.DepositorInsertAt1g04880 terminator
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGC014
Plasmid#48827PurposeProvides GFP as GreenGate coding sequence module.DepositorInsertGFP
UseGolden gate compatible cloning vectorTagsExpressionMutationA206KPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGC024
Plasmid#48829PurposeProvides BFP as GreenGate coding sequence module.DepositorInsertBFP
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-hEF1A-MG-U6-sgRNA
Plasmid#175505PurposeAll-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhEF1a, U6Available sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV GG hUbC-dsRED
Plasmid#84034PurposeContains Golden Gate cassette to express up to four gRNAs and dsREDDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox_v2
Plasmid#198040PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 kanR cassette
Plasmid#117122PurposeKanamycin resistance cassetteDepositorInsertKanamycin resistance cassette for Bacillus subtilis selection
UseGolden gate donor vector for gene targeting in ba…TagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only