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36INS-J23100_GB

Plasmid

#78669

Purpose

36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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36INS-J23100_FB

Plasmid

#78668

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
36INS-J23100_EB

Plasmid

#78667

Purpose

36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
36INS-J23100_AB

Plasmid

#78666

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated J23100 Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTB-Multibow-mO

Plasmid

#60989

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKO

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

mKO

Use

Zebrafish

Tags

membrane tag

Expression

Mutation

Promoter

Bactin2

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTB-Multibow-mY

Plasmid

#60988

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound citrine

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

citrine

Use

Zebrafish

Tags

membrane tag

Expression

Mutation

Promoter

Bactin2

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pUAST myc mouse Rab35 CA

Plasmid

#53516

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Tags

myc

Expression

Insect

Mutation

Q67L

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pEPOR0SP0016

Plasmid

#117526

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.0

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.0 (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

K810A, K1003A, R1060A

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDM344

Plasmid

#108974

Purpose

shuttle vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

Expression

Mutation

Promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-EPN-07

Plasmid

#79922

Purpose

Palm1-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-07

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 (1-428)

Plasmid

#60395

Purpose

Expression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

Expression

Yeast

Mutation

deleted 429 - end

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-20

Plasmid

#79935

Purpose

Late2-4GS-I3-01-myc-PH

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-20

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01

Plasmid

#79916

Purpose

Myr1-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-hO

Plasmid

#60996

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear mKO

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

mKO

Use

Zebrafish

Tags

histone 2B tag

Expression

Mutation

Promoter

Bactin2

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-mC

Plasmid

#60986

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound cerulean

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

Cerulean

Use

Zebrafish

Tags

membrane tag

Expression

Mutation

Promoter

Bactin2

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pSALECT-Ham1

Plasmid

#59706

Purpose

pSALECT with Ham1 insert

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

Ham1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST tdTomato Rab35 DN

Plasmid

#53499

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Use

Tags

tdtomato

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUAST tdTomato Rab35 WT

Plasmid

#53502

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Use

Tags

tdtomato

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP Rab35 DN

Plasmid

#53501

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Use

Tags

YFP

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only