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pSAVED-CHAT_PCaspase-N-C-term

Plasmid

#214046

Purpose

Bacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Use

Tags

Expression

Bacterial

Mutation

aa 1-153, 154-666

Promoter

lacUV5 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT-sm_PCaspase

Plasmid

#214048

Purpose

Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Use

Tags

Expression

Bacterial

Mutation

R456E, D459K, D476K, R479E

Promoter

lacUV5 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-Aurora B KD

Plasmid

#184043

Purpose

Expresses H2B-GFP fused to Aurora B kinase dead

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

AURKB (AURKB Human)

Use

Tags

EGFP and H2B

Expression

Mammalian

Mutation

Changed Lysine 65 to Arginine

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2575

Plasmid

#144045

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HSF5 (HSF5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2313

Plasmid

#142404

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ETV3 (ETV3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD1B-COMP5AP-AviTag-9xHis

Plasmid

#157404

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD1B (CD1B Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF4L2_p.P160L

Plasmid

#81404

Purpose

Gateway Donor vector containing DCAF4L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DCAF4L2 (DCAF4L2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P160L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-hIKAROS-IRES-GFP

Plasmid

#74046

Purpose

retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Ikaros (IKZF1) (IKZF1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-MYO5C

Plasmid

#135404

Purpose

Expresses GFP-tagged human MYO5C in mammalian cells.

Depositor

Richard Cheney

Article

Jacobs et al Mol Biol Cell. 2009 Nov;20(21):4471-88.

Insert

MYO5C (MYO5C Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Relative to the GenBank MYO5C cDNA sequence repor…

Promoter

CMV

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-hHelios-IRES-GFP

Plasmid

#74047

Purpose

retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Helios (IKZF2) (IKZF2 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

encodes shorter version of human Helios

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-E55A-ATPIF1

Plasmid

#85404

Purpose

Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase

Depositor

David Sabatini

Article

Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

Insert

ATPIF1 (ATP5IF1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

E55A point mutation abrogating the ability of ATP…

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
TFORF2594

Plasmid

#144048

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

BHLHE41 (BHLHE41 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-NSP5

Plasmid

#154404

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

July 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAP4K3 gRNA (BRDN0001145375)

Plasmid

#76404

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K3 (MAP4K3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-muGFP-hATG2A ΔWIR

Plasmid

#215507

Purpose

Expresses GFP tagged human ATG2A with the mutation in WIPI interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2587

Plasmid

#144047

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CERS5 (LASS5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-861_R

Plasmid

#147404

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-861

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-861 (gw Fly, Nematode)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_L4139P-eGFP-3HA

Plasmid

#108404

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Human)

Use

Tags

3xHA and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-LucA

Plasmid

#174047

Purpose

Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinase

Depositor

Tyler Jacks

Article

Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

Insert

LucA

Use

Cre/Lox, Lentiviral, and Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

ALG8 alanine 506 to threonine

Promoter

Human Ubiquitin C

Available since

Availability

Academic Institutions and Nonprofits only

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Lenti-LucL

Plasmid

#174048

Purpose

Expresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinase

Depositor

Tyler Jacks

Article

Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

Insert

LucL

Use

Cre/Lox, Lentiviral, and Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

LAMA4 glycine 1254 to valine

Promoter

Human Ubiquitin C

Available since

Availability

Academic Institutions and Nonprofits only

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