-
Plasmid#139346PurposeExpression of kinase-dead TYK2-K930R proteinDepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-K930RPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC30X-BS(X)
Plasmid#58958PurposeEncodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertEnhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
UseTagsFirst 8 codons of COX3ExpressionMutationGFP modified as described: CORMACK, B. P., R. H. …Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
mADAM17deltaMP in pQCXIP cFLAG
Plasmid#19144DepositorInsertADAM17 (Adam17 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationdelta 189-378PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pMKO.1-shMki67
Plasmid#160958PurposeMki67 shRNA in pMKO.1 retroviral vectorDepositorInsertMki67 (Mki67 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE
Plasmid#140230PurposeDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFPDepositorInsertLuciferase + crIFNg (reverse orientation)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
GCN2-KD1-pGEX4T-1
Plasmid#21876DepositorInsertGCN2 (Eif2ak4 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Bbs4
Plasmid#128868PurposeFor mammalian expression of mouse Bbs4DepositorInsertBbs4 (Bbs4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SPN_ARR wt (Shank3)
Plasmid#175254PurposeSPN-ARR fragment in EGFP backbone for mammalian expressionDepositorInsertSPN-ARR fragment from SHANK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET42b-A3A-PBE-ΔUGI
Plasmid#119771PurposeTo express the recombinant APOBEC3A in vitroDepositorInsertAPOBEC3A-nCas9-NLS
UseTags6*HIS and NLSExpressionBacterialMutationnCas9 has a mutation D10A in wild type SpCas9PromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE-ΔUGI
Plasmid#119770PurposeConversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)DepositorInsertAPOBEC3A-nCas9-ΔUGI-NLS
UseTagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 STAT3
Plasmid#67120PurposeStandard for cell-free expression benchmarking.DepositorInsertSTAT3 (STAT3 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2V362F-VSV
Plasmid#139345PurposeExpression of TYK2 V362F protein (rs2304256)DepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362FPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEH305 HA NLS SpCas9 3x high affinity MS2 loops
Plasmid#168216PurposeMammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tagDepositorInsertSV40 NLS SpCas9
UseLentiviralTagsHA, SV40 NLS, and nucleoplasmin NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p27(kip1)
Plasmid#50026PurposepENTR vector for human cyclin-dependent kinase inhibitor 1BDepositorInsertp27(kip1) (CDKN1B Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shPrc1
Plasmid#160959PurposePrc1 shRNA in pMKO.1 retroviral vectorDepositorInsertPrc1 shRNA (Prc1 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-848-952(pL91)
Plasmid#124388PurposeE.Coli produced TET1-848-952 purified by FPLC nickel columnDepositorInsertGB1-His-TET1-848-952 wildtype (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-SAP25-126-186(pL91)
Plasmid#124387PurposeE.Coli produced SAP25 SID purified by FPLC nickel columnDepositorInsertGB1-His-SAP25-126-186 (SAP25 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-BE3-2NLS
Plasmid#119031PurposeStably express BE3 in mammalian cellsDepositorInsertBE3
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only