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pKH118_keima-YIPF4

Plasmid

#214971

Purpose

Golgiphagy flux reporter keima-YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

keima-YIPF4 (YIPF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-sgRNA-v2

Plasmid

#204639

Purpose

Expression of trancated AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

trancated AsCas12f sgRNA (sgRNA-v2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Flag p21 WT

Plasmid

#16240

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9

Plasmid

#47106

Purpose

Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Tags

Flag and SV40 NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNAFLAG-ATM-kd

Plasmid

#43908

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

ataxia telangiectasia mutated (ATM Human)

Use

Tags

FLAG and His

Expression

Mammalian

Mutation

kinase dead (D2870A and N2875K)

Promoter

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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BB3cK_pGAP_23*_pLAT1_Cas9

Plasmid

#104908

Purpose

hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe4_eGFP

Plasmid

#221463

Purpose

AAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pCK122 pDESTattB_exorh:mCherry

Plasmid

#213511

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al bioRxiv 2023.12.08.570868

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK522-FAST-PETase

Plasmid

#214754

Purpose

plasmid for expression FAST-PETase in Pseudomonas putida

Depositor

Hal Alper

Article

Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

Insert

FAST-PETase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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