166,224 results

1. pGL4.10/RSV_SF3B3-miniXon_Luciferase

   Plasmid

   #174660

   Purpose

   Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.

   Depositor

   Beverly L. Davidson

   Article

   Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

   Insert

   miniXon cassette
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Kozak ATG initiation sequence in middle exon

   Promoter

   RSV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 HA Arf1 DN T31N

   Plasmid

   #10833

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf1 T31N (ARF1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T31N. Defective in GTP binding. Dominant negati…

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPRIME-CMV-dsRed-FF3

   Plasmid

   #11664

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of dsRed and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   dsRed

   Expression

   Mammalian

   Available Since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pLKO5d.EFS.SpCas9.P2A.PAC

   Plasmid

   #58329

   Purpose

   Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   EFS (EEF1A1 Human)
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. mClY-N1

   Plasmid

   #90457

   Purpose

   A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

   Insert

   mClY-N1
   

   Expression

   Mammalian

   Mutation

   EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD268

   Plasmid

   #132523

   Purpose

   mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Daniel Dickinson, Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mNG-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized mNG

   Expression

   Worm

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP1830

   Plasmid

   #70708

   Purpose

   Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Insert

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   CAG

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVTNT-T7-KPNA6

   Plasmid

   #26682

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 6 (KPNA6 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCardinal-N1

   Plasmid

   #54590

   Purpose

   Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Type

   Empty backbone

   Tags

   mCardinal

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pG-dsRedxp-HIF1Beta

    Plasmid

    #87213

    Purpose

    N-terminally dsRedXP-tagged HIF-1b/ARNT

    Depositor

    Violaine Sée

    Insert

    HIF-1b (ARNT Human)
    

    Tags

    dsRedXP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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