166,806 results

1. pExp-NusA

   Plasmid

   #112571

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8His and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0815

   Plasmid

   #141717

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LHX2 (LHX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.GFAP.Cre.WPRE.hGH

   Plasmid

   #105550

   Purpose

   AAV expression of Cre from GFAP promoter

   Depositor

   James M. Wilson

   Has Service

   AAV PHP.eB and AAV5

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   GFAP

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cox8x2-OeNL_pcDNA3

   Plasmid

   #89526

   Purpose

   Orange color variant of bright luminescent protein, targeted to the Mitochondria

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   OeNL
   

   Tags

   CoxVIII X2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGD000

   Plasmid

   #48859

   Purpose

   Empty entry vector for creating GreenGate C-terminal tag modules.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Type

   Empty backbone

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

   Plasmid

   #139987

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. POLG-pcDNA3.1

   Plasmid

   #191487

   Purpose

   Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.

   Depositor

   Hans Zempel

   Insert

   POLG (POLG Human)
   

   Tags

   V5 tag, 6X His

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+_Lambda-N-HA-Peptide

   Plasmid

   #92005

   Purpose

   Expresses Lambda-N-HA peptide

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   Lambda-N-HA peptide
   

   Tags

   Lambda-N-HA

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lck-mCherry-RGS

   Plasmid

   #112935

   Purpose

   Plasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Mol Biol Cell. 2017 Nov 7;28(23):3371-33

   Insert

   p115RhoGEF-RGS domain
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid

    #101178

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/H698A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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