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pLenti-Sa-gRNA-puro

Plasmid

#191652

Purpose

Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

S. aureus gRNAscr

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLenti-eGFP-dSaCas9-blast

Plasmid

#191653

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

eGFP-T2A-dSaCas9

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLenti-dSaCas9-KRAB-blast

Plasmid

#191654

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

dSaCas9-KRAB

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1

Plasmid

#191675

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A

Depositor

Veerle Baekelandt

Inserts

ATP10B (ATP10B Human)
TMEM30A (TMEM30A Human)

Use

Lentiviral

Tags

6X his and flag

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A

Plasmid

#191676

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A

Depositor

Veerle Baekelandt

Inserts

ATP10B (ATP10B Human)
TMEM30A (TMEM30A Human)

Use

Lentiviral

Tags

6x HIS and flag

Expression

Mutation

D433N

Promoter

Availability

Academic Institutions and Nonprofits only

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TRE-Lepr_Short-IRES-eGFP

Plasmid

#196935

Purpose

Doxycycline inducible expression of short Lepr (no signaling) with eGFP marker

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

Lepr (Lepr Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

short isoform, lack of signaling casacade

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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SBE-NLS-mCherry-P2A-CreERT2 PGK-rtTA3

Plasmid

#196936

Purpose

TGFbeta reporter driven nuclear mCherry and CreERT2 with PGK driven rtTA3

Depositor

Elaine Fuchs

Article

Oshimori et al Cell. 2015 Feb 26;160(5):963-976. doi: 1

Insert

mCherry

Use

Lentiviral and RNAi

Tags

P2A-CreERT2

Expression

Mammalian

Mutation

Promoter

SBE

Availability

Academic Institutions and Nonprofits only

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FUGW-3xHA-KCNJ6-mCherry

Plasmid

#197032

Purpose

Express 3xHA-tagged human KCNJ6 (Girk2), co-expressed with mCherry, from UbiC promoter

Depositor

Ronald Hart

Insert

KCNJ6 (KCNJ6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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FSW-tdTomato

Plasmid

#197033

Purpose

Express tdTomato from hSyn1 promoter

Depositor

Ronald Hart

Insert

tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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FSW_jGCaMP8f

Plasmid

#197034

Purpose

Express GCaMP8f from hSyn1 promoter

Depositor

Ronald Hart

Insert

GCaMP8f

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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FUGW-APOBEC1-YTH-mCherry

Plasmid

#197035

Purpose

Express APOBEC1 cytosine deaminase fused with YTH m6A binding domain from UbiC promoter.

Depositor

Ronald Hart

Insert

APOBEC1-YTH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-T2A-EGFP

Plasmid

#197036

Purpose

Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2 (ALDH2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-E504K-T2A-EGFP

Plasmid

#197037

Purpose

Express ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2-E504K

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E504K

Promoter

Availability

Academic Institutions and Nonprofits only

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yWL268 MCP-GFP

Plasmid

#197052

Purpose

pUBC-NLS-HA-2xMCP-2xGFP

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-2xGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

ubc

Availability

Academic Institutions and Nonprofits only

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yWL229 MCP-GFP-eRF3

Plasmid

#197053

Purpose

pUBC-NLS-HA-2xMCP-1xGFP-eRF3

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-GFP-eRF3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

ubc

Availability

Academic Institutions and Nonprofits only

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yWL234 MCP-GFP-PABPC1*

Plasmid

#197054

Purpose

pUBC-NLS-HA-2xMCP-1xGFP-PABPC1*

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-GFP-PABPC1*

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

ubc

Availability

Academic Institutions and Nonprofits only

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pLKO-TRE-AFOS

Plasmid

#197079

Purpose

A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

Depositor

Elaine Fuchs

Article

Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

Insert

AFOS (Fos Mouse)

Use

Lentiviral

Tags

Flag and T7

Expression

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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pLV-CS-060

Plasmid

#197133

Purpose

CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Type

Empty backbone

Use

Lentiviral

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

MCP(N55K)

Promoter

Availability

Academic Institutions and Nonprofits only

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pLV-CS-117

Plasmid

#197134

Purpose

CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CS-119

Plasmid

#197135

Purpose

CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al bioRxiv 2023.01.18.524633

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge