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Plasmid#188589PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc and GWpep mmExpressionInsectMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K276A]
Plasmid#188597PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK276A [AAG=>GCG]PromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]-Gwpep(Dm)
Plasmid#188590PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc and Gwpep dmExpressionInsectMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Gwpep(Dm)-Nbr[C399-625]
Plasmid#188592PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc-Gwpep dmExpressionInsectMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Gwpep(Mm)-Nbr[C399-625]
Plasmid#188591PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc-Gwpep mmExpressionInsectMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A]
Plasmid#188596PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K284A]
Plasmid#188595PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK284A [AAG=> GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A]
Plasmid#188594PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K187A]
Plasmid#188593PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK187A [AAA=> GCA]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pILGFP4D41
Plasmid#185841PurposeTesting the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B11)
Plasmid#185859PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV492
Plasmid#177700PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV491
Plasmid#177699PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV488
Plasmid#177696PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast speciesDepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterTDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSN071
Plasmid#166729PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1-NLS
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN072
Plasmid#166730PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1-NLS
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM0649
Plasmid#129010PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM0650
Plasmid#129011PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0648
Plasmid#129009PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only