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DMP1-GFPtopaz

Plasmid

#114270

Purpose

GFP labeling of mature osteoblasts and osteocytes.

Depositor

David Rowe

Article

Kalajzic et al Bone. 2004 Jul;35(1):74-82. doi: 10.1016

Insert

Dmp1 + GFPtopaz (Dmp1 Mouse, Aequorea victoria)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dmp1

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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Lox-stop-Lox p53 R270H

Plasmid

#14853

Depositor

Tyler Jacks

Article

Olive et al Cell. 2004 Dec 17. 119(6):847-60.

Insert

p53 R270H (Trp53 Mouse)

Use

Cre/Lox

Tags

Lox-STOP-lox

Expression

Mammalian

Mutation

Contact mutant R270H. (Murine p53 codon 270 corre…

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
PG13-gal (wt p53 binding sites)

Plasmid

#16446

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
Neurod1-Atoh7HA3

Plasmid

#44438

Depositor

William Klein

Article

Mao et al Development. 2013 Feb;140(3):541-51. doi

Insert

Atoh7-3xHA knock-in (Atoh7 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Neurod1 left arm, Neurod1 right arm, and loxC2-Re…

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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MG15-gal (mut p53 binding sites)

Plasmid

#16447

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (mutant) (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBS NL4-3 envFS eGFP 5'CMV Δtat

Plasmid

#101347

Purpose

tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta Tat

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDP1.2

Plasmid

#171092

Purpose

Expresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)

Depositor

Dario Palmieri

Article

Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

Insert

AAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1

Use

Synthetic Biology

Tags

Emerald, Geminin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-CNL

Plasmid

#65712

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-ONL

Plasmid

#65713

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, ONL

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-tRNA-Ser

Plasmid

#174318

Purpose

For in vitro transcription of mouse tRNAs

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

tRNA-ser

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-AMV-α1L9'S-nAchR

Plasmid

#20684

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrna1 Mouse)

Use

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Promoter

Available since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pBSK-SN3 (wt p53)

Plasmid

#16421

Depositor

Bert Vogelstein

Article

Baker et al Science. 1989 Apr 14. 244(4901):217-21.

Insert

p53 (TP53 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
D3 clone (p21/WAF1 promoter)

Plasmid

#16452

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 promoter (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pCA-G-intron(Neo)-T(FLLFLNL)

Plasmid

#40020

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdT-3Myc
beta-globin intron
Neo

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pBSSK p85 alpha (delta SH3, iSH2)

Plasmid

#13433

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta SH3, iSH2) (PIK3R1 Human)

Use

Tags

Flag

Expression

Bacterial

Mutation

Flag tags replacing both the SH3 and iSH2 domains.

Promoter

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pBS human Frataxin (myc tag)

Plasmid

#11443

Depositor

Ronald Kahn

Article

Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Frataxin (FXN Human)

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pBT250_(pCA-G-intron(Neo)-tTA2)

Plasmid

#36876

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pBS-AMVpl-β1WT-nAchR

Plasmid

#97080

Purpose

To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

Beta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-YNL

Plasmid

#65711

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, YNL

Use

Luciferase; Tol2 system

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK+ chickBMPR-1B DN

Plasmid

#49529

Purpose

cloning vector for dominant negative chick BMP receptor 1B

Depositor

Peter ten Dijke, Lee Niswander

Article

Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

Insert

BMPR-1B DN (BMPR1B Chicken)

Use

Tags

Expression

Bacterial

Mutation

dominant negative mutation (K231R); also P2A, M11…

Promoter

T3

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only