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Plasmid#193021PurposehnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP C RRMExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RBD#3L
Plasmid#193020PurposehnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
UseTagshnRNP A1 RRM2ExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS
Plasmid#194750PurposeTo fuse your protein of interest to the N-terminus of GPI-anchored-BioID2DepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CALID
Plasmid#169708PurposeCre-activated light-inducible Dre recombinaseDepositorInserta double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
PC3-709-33-10_GAL4-FKBP12(F36V)
Plasmid#200438PurposeExpresses the GAL4 DNA Binding domain linked to the FKBP12(F36V) target proteinDepositorInsertGAL4-FKBP12(F36V)
UseTagsGAL4, FKBP12(F36V)ExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-BBclone1
Plasmid#137069PurposeE. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238DepositorInsertsUseTagsExpressionBacterialMutationwild-type, full-length (signal-peptide-containing…PromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ABE9
Plasmid#194208PurposeMammalian expression of ABE9DepositorInsertTadA-8e (N108Q/L145T)-nSpCas9
UseCRISPRTags6*HisExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Td-CGBE
Plasmid#196599PurposeMammalian expression of Td-CGBEDepositorInsertbpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
UseTags6*HisExpressionMammalianMutationTadA-8e (N46L); S. pyogenes Cas9 (D10A)PromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-EYFP-WPRE-bGHpA
Plasmid#213971PurposeAAV expression of EYFP from pCALM1 promoterDepositorInsertpCALM1
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MbPylRS(DiZPK)
Plasmid#91706Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interestDepositorInsertspylS
pylT
UseTagsExpressionMammalianMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257APromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-N108Q
Plasmid#194157PurposeMammalian expression of ABE8e-N108QDepositorInsertTadA-8e (N108Q)-nSpCas9
UseCRISPRTags6*HisExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV2-hSyn-G-Flamp1
Plasmid#188569Purposegreen biosensor for cAMP in neuronsDepositorInsertG-Flamp1
UseAAVTagsHis tagExpressionMutationPromoterhSynAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp1-mut
Plasmid#188568Purposecontrol probe of G-Flamp1, insensitive to cAMPDepositorInsertG-Flamp1-mut
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-CBE
Plasmid#196293Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GPDepositorInsertFull length TSWV M antigenome encoding CBE in place of viral GP
UseCRISPRTags3×flagExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV2-hSyn-G-Flamp1-mut
Plasmid#188570Purposecontrol probe of G-Flamp1, insensitive to cAMPDepositorInsertG-Flamp1-mut
UseAAVTagsHis tagExpressionMutationPromoterhSynAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 S135A
Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationS135APromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS
Plasmid#194751PurposeTo fuse your protein of interest to the C-terminus of GPI-anchored-BioID2DepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
eNS2B47NS3 S135A
Plasmid#155318PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationS135APromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3
Plasmid#213973PurposeAAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locusDepositorInsertShank3 sgRNA
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only