Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 1261 - 1280 of 4601 results

  3. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Availability
    Academic Institutions and Nonprofits only
    87844-plasmid-map-sequence-id-174620

    Enlarge

  4. pFC332

    Plasmid
    #87845
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Availability
    Academic Institutions and Nonprofits only
    87845-plasmid-map-sequence-id-418925

    Enlarge

  5. pAC1430

    Plasmid
    #127981
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Availability
    Academic Institutions and Nonprofits only
    127981-plasmid-map-sequence-id-254494

    Enlarge

  6. pAC1748

    Plasmid
    #127982
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Availability
    Academic Institutions and Nonprofits only
    127982-plasmid-map-sequence-id-254497

    Enlarge

  7. pAC1749

    Plasmid
    #127983
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Availability
    Academic Institutions and Nonprofits only
    127983-plasmid-map-sequence-id-254502

    Enlarge

  8. pAC1750

    Plasmid
    #127984
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Availability
    Academic Institutions and Nonprofits only
    127984-plasmid-map-sequence-id-254510

    Enlarge

  9. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Use
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

    Enlarge

  10. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

    Enlarge

  11. pX330-Flag-deSpCas9

    Plasmid
    #92114
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive eSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A, K848A, K1003A, R1060A
    Promoter
    Cbh
    Availability
    Academic Institutions and Nonprofits only
    92114-plasmid-map-sequence-id-197615

    Enlarge

  13. pX330-Flag-dHeFSpCas9

    Plasmid
    #92116
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    Cbh
    Availability
    Academic Institutions and Nonprofits only
    92116-plasmid-map-sequence-id-177904

    Enlarge

  15. HeFSpCas9

    Plasmid
    #92355
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Availability
    Academic Institutions and Nonprofits only
    92355-plasmid-map-sequence-id-197617

    Enlarge

  18. rnALDH

    Plasmid
    #67920
    Purpose
    Expresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Rattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
Showing: 1261 - 1280 of 4601 results