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Showing: 1261 - 1280 of 2066 results

  8. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid
    #108244
    Purpose
    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression
    Depositor
    Jerome Bonnet
    Article
    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102
    Insert
    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    pLacO1
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108244-plasmid-map-sequence-id-210116

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  11. pS148∙CsR

    Plasmid
    #197858
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Article
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Insert
    gRNA scaffold - Ap/Cb resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197858-plasmid-map-sequence-id-393255

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  15. pHyg-AID*

    Plasmid
    #99515
    Purpose
    C-terminal AID* degron cassette with HygR marker for S. cerevisiae
    Depositor
    Helle Ulrich
    Article
    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10
    Insert
    IAA17(71-114) (AXR3 Mustard Weed)
    Use
    Pcr template for yeast tagging cassette
    Tags
    Expression
    Mutation
    internal inversion of nucleotides 265-312 of the …
    Promoter
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99515-plasmid-map-sequence-id-191042

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  18. ND4.2-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #179682
    Purpose
    Expression of base editor in mammalian cells and for cell sorting
    Depositor
    David Liu
    Article
    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387
    Insert
    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S1330I A1341V N1342S E1370K T1380I
    Promoter
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179682-plasmid-map-sequence-id-355098

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  20. CMV-R-GECO1

    Plasmid
    #32444
    Purpose
    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 8.
    Insert
    R-GECO1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32444-plasmid-map-sequence-id-117093

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Showing: 1261 - 1280 of 2066 results