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Showing: 1261 - 1280 of 22673 results

  1. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CBH
    Availability
    Academic Institutions and Nonprofits only
    200389-plasmid-map-sequence-id-407168

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  2. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid
    #200910
    Purpose
    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    3XHA_den1FnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Changed Glutamic Acid to Arginine at 1369 position
    Promoter
    CBH
    Availability
    Academic Institutions and Nonprofits only
    200910-plasmid-map-sequence-id-407164

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  4. pS-cr:GFP

    Plasmid
    #196295
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    SpeI-DR-BsaI-DR-SpeI
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Availability
    Academic Institutions and Nonprofits only
    196295-plasmid-map-sequence-id-390035

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  5. pS-sg:GFP

    Plasmid
    #196296
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Availability
    Academic Institutions and Nonprofits only
    196296-plasmid-map-sequence-id-389821

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  12. LLP793_L2_V05_s35S_AND_GFP_pOp6_Flp_CO2_B3

    Plasmid
    #192397
    Purpose
    To test if a single plasmid stably transformed into Arabidopsis can switched from off to on when its AND gate unit is activated by both cell type (cortex) and chemical induction (by DEX).
    Depositor
    Ryan Lister
    Article
    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038
    Insert
    35S::FRT-OCS-FRT::B3RT-OCS-B3RT::Turbo GFP
    Use
    Synthetic Biology
    Tags
    N7
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    192397-plasmid-map-sequence-id-378296

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  16. pINDUCER20_mGFP

    Plasmid
    #190027
    Purpose
    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mGFP
    Depositor
    Andrew Tutt
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    190027-plasmid-map-sequence-id-377385

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Showing: 1261 - 1280 of 22673 results