Addgene

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pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

Plasmid

#187959

Purpose

Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Inserts

Blasticidin resistance
FKBP12_F36V

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

Plasmid

#92139

Purpose

Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMH0004

Plasmid

#174087

Purpose

UCOE-SFFV-Cas9-BFP

Depositor

Jonathan Weissman

Article

Hein et al Nat Biotechnol. 2022 Mar;40(3):391-401.

Insert

Cas9-BFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

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pMH0001

Plasmid

#85969

Purpose

UCOE-SFFV-dCas9-BFP-KRAB

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Insert

dCas9

Use

Lentiviral

Tags

BFP-KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

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pMCB619

Plasmid

#171011

Purpose

CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

U6:sgRNA_Puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Availability

Academic Institutions and Nonprofits only

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pRT029

Plasmid

#127969

Purpose

double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pJH3458

Plasmid

#179573

Purpose

Pttr-39::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans D-MN -specific expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

BFP

Expression

Worm

Mutation

Promoter

Pttr-39

Availability

Academic Institutions and Nonprofits only

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pJH3449

Plasmid

#179576

Purpose

Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

BFP

Expression

Worm

Mutation

Promoter

Plgc-55B

Availability

Academic Institutions and Nonprofits only

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pJH3453

Plasmid

#179577

Purpose

Pglc-55B::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVB/others premotor IN and others expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

BFP

Expression

Worm

Mutation

Promoter

Plgc-55B

Availability

Academic Institutions and Nonprofits only

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pJH3456

Plasmid

#179581

Purpose

Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

BFP

Expression

Worm

Mutation

Promoter

Pnmr-1

Availability

Academic Institutions and Nonprofits only

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pMH0007

Plasmid

#174162

Purpose

UCOE-EF1A-Cas9-BFP

Depositor

Jonathan Weissman

Article

Hein et al Nat Biotechnol. 2022 Mar;40(3):391-401.

Insert

Cas9-BFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

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pGGC024

Plasmid

#48829

Purpose

Provides BFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

BFP

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTK511

Plasmid

#114686

Purpose

Express Mem-BFP-iLID

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

Mem-BFP-iLID

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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2SP8

Plasmid

#126533

Purpose

2-input history dependent program A then B;BFP-0-sfGFP

Depositor

Jerome Bonnet

Article

Zuniga et al Nat Commun. 2020 Sep 21;11(1):4758. doi:

Insert

2-input A then B;BFP-0-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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2SP9

Plasmid

#126534

Purpose

2-input history dependent program A then B;BFP-mKate2-sfGFP

Depositor

Jerome Bonnet

Article

Zuniga et al Nat Commun. 2020 Sep 21;11(1):4758. doi:

Insert

2-input A then B;BFP-mKate2-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
3SP1

Plasmid

#126535

Purpose

3-input history dependent program 0-BFP-mKate2-sfGFP

Depositor

Jerome Bonnet

Article

Zuniga et al Nat Commun. 2020 Sep 21;11(1):4758. doi:

Insert

0-BFP-mKate2-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
3SP3

Plasmid

#126537

Purpose

3-input history dependent program 0-0-0-BFP

Depositor

Jerome Bonnet

Article

Zuniga et al Nat Commun. 2020 Sep 21;11(1):4758. doi:

Insert

0-0-0-BFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
3SP5

Plasmid

#126539

Purpose

3-input history dependent program 0-BFP-0-sfGFP

Depositor

Jerome Bonnet

Article

Zuniga et al Nat Commun. 2020 Sep 21;11(1):4758. doi:

Insert

0-BFP-0-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GXB

Plasmid

#160926

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GYB

Plasmid

#160927

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Availability

Academic Institutions and Nonprofits only

Enlarge