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pPBT-PE2-PGK-Blast

Plasmid

#173219

Purpose

piggyBac transposon containing PE2 and Blasticidin expression cassettes

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

PE2
Blast

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

CMV and hPGK

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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LZF97_hSyn-spine-jRGECO1a

Plasmid

#119198

Purpose

Targets jRGECO1a to spines in living neurons.

Depositor

Don Arnold

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L) (Utrn Synthetic, Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human Synapsin 1

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-mTurquoise2-NLS

Plasmid

#165443

Purpose

Expresses nuclear mTurquoise2 from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

mTurquoise2-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKK233-hCaM

Plasmid

#47598

Depositor

Emanuel Strehler

Article

Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

Insert

CaM (CALM3 Human)

Use

Tags

Expression

Bacterial

Mutation

contains 469 bp fragment (nt 92-559)

Promoter

trc

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pIDTSmart-MbPylRS

Plasmid

#217361

Purpose

Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

M. barkeri pyrrolysyl-tRNA synthetase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pTiGc

Plasmid

#78314

Purpose

Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).

Depositor

Samantha Sampson

Article

Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/

Inserts

TurboFP635
GFP

Use

Synthetic Biology; Mycobacterial replicating vect…

Tags

Expression

Bacterial

Mutation

Promoter

hsp60 and hsp60(ribo)

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBJ1 neo-hu FceRI gamma

Plasmid

#16540

Depositor

Jean Kinet

Article

Kuster et al J Biol Chem 1990 Apr 15;265(11):6448-52.

Insert

FCER1G (FCER1G Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pPBT-peRNA_GG-Puro

Plasmid

#173220

Purpose

piggyBac transposon containing hU6 promotor and pegRNA cloning site

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

pegRNA cloning cassette
PuroR

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a and hU6

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pUAS-Ncad-GFP

Plasmid

#35986

Depositor

James Jontes, Stephen Smith

Article

Jontes et al J Neurosci. 2004 Oct 13;24(41):9027-34.

Insert

Ncad (cdh2 Zebrafish)

Use

Gal4 inducible

Tags

EGFP

Expression

Mutation

Promoter

14xUAS-E1b

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-PKCg-mEGFP-N1

Plasmid

#112270

Purpose

IDOCKSgamma Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c gamma (Prkcg Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1 GSK3beta S9A HA

Plasmid

#15994

Depositor

Morris Birnbaum

Article

Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

Insert

GSK3beta (GSK3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S9A: constitutively active

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pCAGGS-FLAG-BMAL1-S513A

Plasmid

#186831

Purpose

Expresses FLAG-BMAL1-S513A in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 513 to Alanine

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1-S515A

Plasmid

#186832

Purpose

Expresses FLAG-BMAL1-S515A in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 515 to Alanine

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1-S516A

Plasmid

#186833

Purpose

Expresses FLAG-BMAL1-S516A in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 516 to Alanine

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1-S519A

Plasmid

#186834

Purpose

Expresses FLAG-BMAL1-S519A in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 519 to Alanine

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_Z3_Gal4VP64

Plasmid

#169916

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine

Plasmid

#104122

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

spine-jRGECO1a-P2A-QuasAr-dark Citrine

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRFb-DN-GFP

Plasmid

#37111

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRFb-DN-GFP (ptprfb Zebrafish)

Use

Tol2 gateway expression vector

Tags

EGFP

Expression

Mutation

Deleted aa1386-1909 corresponding to phosphatase …

Promoter

10xUAS

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn grpLight1.2

Plasmid

#175174

Purpose

genetically encoded GRP sensor grpLight1.2

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

hSyn-driven grpLight1.2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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