-
Plasmid#215258PurposeModule for the constitutive expression of the N. nambi CPH, LUZ, H3H genes, EGFP and p19 fused to a SF in alpha2.DepositorInsertSF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4468
Plasmid#215257PurposeModule for the constitutive expression of the N. nambi LUZ, H3H genes, EGFP and p19 fused to a SF in omega2.DepositorInsertSF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4665
Plasmid#215270PurposeModule for the expression of nptII and the N. nambi LUZ and CPH genes flanked by insulators on the sides of the construction.DepositorInsertMAR10-SF-nptII-LUZ-CPH-MAR10-SF
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4666
Plasmid#215271PurposepUPD2 for the Arabidopsis thaliana ABA-responsive MAPKKK18 promoter.DepositorInsertpromMAPKKK18
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3474
Plasmid#215370PurposeCDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathwayDepositorInsertCPH
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4193
Plasmid#215251PurposeTU for the expression of the CIB1 gene fused to the VPR activation domain under the P35S promoter and TNOS terminator in alpha1DepositorInsertP35S:CIB1:VPR:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3473
Plasmid#215246PurposeCDS of Luz gene from N. nambi codon optimized for N.benthamiana. This gene generate the luminiscence of the bioluminiscente pathwayDepositorInsertLuz
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3472
Plasmid#215245PurposeCDS of H3H from N. nambi codon optimized for N.benthamiana. This gene is the second step of the bioluminiscente pathwayDepositorInsertH3H
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL686
Plasmid#207510PurposeePPE for prime editing in riceDepositorInsertzCas9 (H840A)-NC-M-MLV RT-deltaRH
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-RBCS-tagRFP
Plasmid#213868PurposeBinary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertRIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-mCherry
Plasmid#213862PurposeBinary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused mCherry-UnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGA-pCLV3
Plasmid#210528PurposeCloning A. thaliana CLV3 promoter as GreenGate module ADepositorInsertpCLV3 (CLV3 Mustard Weed)
UseSynthetic Biology; Golden gate compatible cloning…TagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
GW1-mCherryEA
Plasmid#207867PurposeMammalian expression (cytosol) of the mCherry(I158E/Q160A) mutant as a red fluorescent pH sensorDepositorInsertmCherryEA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
cytoDCIP
Plasmid#193861PurposeEncodes mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.DepositorInsertmCherry-GFP1-10
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2911
Plasmid#161764PurposeMODULE C plasmid compatible with the Voytas Plant Genome Engineering Tool kit with an Agrobacterium promoter rolD expressing the TREX2 exonuclease.DepositorInsertrolD:TREX2
UseTagsExpressionBacterialMutationPromoterrolDAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDRD3:ABC006-C02:C220502
Plasmid#183982PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertTDRD3-anti-TDRD3 FAB (TDRD3 Human)
UseTagsanti-TDRD3 FAB HC: N-terminal STII leader peptid…ExpressionBacterialMutationPromoterAvailable sinceJune 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGM41991
Plasmid#153219PurposeLevel M binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in AgrobacteriumDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCk4
Plasmid#136698PurposeAcceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.DepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOG638
Plasmid#105389PurposeSynthetic biologyDepositorInsertpromotor CaBs3 (1000bp), variant
UseSynthetic BiologyTagsExpressionMutationTALE-binding site; BsaI/ BpiI restriction sites r…PromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG639
Plasmid#105390PurposeSynthetic biologyDepositorInsertpromotor CaBs3 (1000bp), variant
UseSynthetic BiologyTagsExpressionMutationTALE-binding site; BsaI/ BpiI restriction sites r…PromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG142
Plasmid#105397PurposeSynthetic biologyDepositorInsertmYFP (GFP variant) fused to PKI nuclear export signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG143
Plasmid#105398PurposeSynthetic biologyDepositorInsertmYFP (GFP variant) fused to SV40 nuclear localization signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A4010
Plasmid#91055PurposeModule A, Promoter: ZmUbi, Gene: dTaCas9-VP64, Terminator: HSPDepositorInsertdTaCas9-VP64
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A4210
Plasmid#91057PurposeModule A, Promoter: Zmubi, Gene: Csy4-P2A-dTaCas9-VP64, Terminator: HSPDepositorInsertCsy4-P2A-dTaCas9-VP64
UseCRISPRTagsExpressionMutationPromoterZmubiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3801
Plasmid#91053PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VP64, Terminator: HSPDepositorInsertCsy4-P2A-dAtCas9-VP64
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBRN168-MXMT
Plasmid#79819PurposeDsREDmonomer-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsRN168/DsREDmonomer (1–168)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_omega1R
Plasmid#68240PurposeGoldenBraid level 1 Omega plant expression vectorDepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_omega2R
Plasmid#68241PurposeGoldenBraid level 1 Omega plant expression vectorDepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
SI13-Lrrc3b
Plasmid#46303DepositorInsertLrrc3b mouse monoclonal antibody (low affinity) (lrrc3b Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDGE347
Plasmid#153228PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_4D5-High-CAR_RHL002
Plasmid#164825PurposeLentiviral vector for constitutive expression of AntiHER2 4D5-High CARDepositorInsertAntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-mono-MAD7
Plasmid#170134PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRTagsExpressionPlantMutationPromotermaize Ubiquitin-1, switchgrass Ubiquitin-1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_4D5-WT-Highest-CAR_RHL004
Plasmid#164826PurposeLentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CARDepositorInsertAntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-PM
Plasmid#183168PurposeConstruct for transient expression of plasma membrane-targeted mCherry in plants.DepositorInsertmCherry-PM
UseTagsOsRac3, O. sativa rac-like GTP-binding protein 3ExpressionPlantMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-Myc
Plasmid#177717PurposeER protein alpha-1-antitrypsin (WT), myc taggedDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsMycExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Mito-mChe
Plasmid#183167PurposeConstruct for transient expression of mitochondrion-targeted mCherry in plants.DepositorInsertMito-mCherry
UseTagsOsCOX11, Oryza sativa cytochrome c oxidase assemb…ExpressionPlantMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_4D5-Low-CAR_RHL001
Plasmid#164824PurposeLentiviral vector for constitutive expression of AntiHER2 4D5-Low CARDepositorInsertAntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM55261
Plasmid#153210PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
35S-VP16-GWY/pAM
Plasmid#201234PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-VP16-GWY
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S-GWY-VP16/pAM
Plasmid#201232PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-GWY-VP16
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE
Plasmid#112872Purposebinary vector for ABE expression in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA cassette
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos
Plasmid#211842PurposePlant transient expression cassette for a Dexamethasone-inducible E3-DART ligaseDepositorInsertsLRR-NEL
GAL4-VP16-GR
UseSynthetic BiologyTagsHA, Myc, and mCherryExpressionPlantMutationPromoter6xUAS-35Smini and pUBQ10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2520
Plasmid#91077PurposeModule B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning
UseCRISPRTagsExpressionMutationPromoterOsU6Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-Flag
Plasmid#58950Purposebackbone for mouse recombinant antibody with added C-terminal FLAG tagDepositorTypeEmpty backboneUseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-EHER
Plasmid#53262PurposeContains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coliDepositorInsertcrtE, idi, crtX, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM65879
Plasmid#153214PurposeLevel 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassetteDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-OsPE2
Plasmid#141080PurposeGateway entry clone for CRISPR-Cas9(H840A) prime editingDepositorInsertzCas9(H840A)-OsM-MLV-RT
UseCRISPR; Gateway compatible zcas9(h840a)-osm-mlv-rtTags3x FLAG, NLS and NLSExpressionPlantMutationPromoterAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shPKM2_4
Plasmid#42516DepositorInsertshRNA-PKM2 (PKM Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-BioLHis
Plasmid#58949Purposebackbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tagDepositorTypeEmpty backboneUseTags6His tag and biotinylation peptideExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2515
Plasmid#91072PurposeModule B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning
UseCRISPRTagsExpressionMutationPromoterAtU6Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only