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Plasmid#136292PurposeExpression of catalytic inactive RNase H fused to micrococcal nucleaseDepositorInsertRNase HI-D10R-E48R and MNase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-D1R
Plasmid#89160PurposeExpress vaccinia virus D1R in mammalian cellsDepositorInsertVaccinia virus D1R
UseTagsExpressionMammalianMutationPromoterCAG promotorAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-LAMP1
Plasmid#207787PurposeExpresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.DepositorInsertsgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_AR-RE_MLPmin_BC1014-luc2
Plasmid#227108PurposeAndrogen receptor response element for luciferase and barcode assays; barcode BC1014DepositorInsert8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-LAMP1
Plasmid#227322PurposeDonor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)DepositorInsertLAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH062
Plasmid#225537PurposeExpresses IS621 in insect cellsDepositorInsertIS621
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_FLuc100---MS2
Plasmid#210574PurposeExpresses Optimal Firefly with 3'UTR MS2 hairpinsDepositorInsertOptimal Firefly luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-LSSmCherry1(-13)
Plasmid#219415PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorArticleInsertLSSmCherry1(-13)
UseTagsMGHHHHHGGExpressionBacterialMutationK137E, K199EPromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherryC1 VAP-A KD/MD
Plasmid#226408PurposeExpression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to mCherry in mammalian cellsDepositorInsertVAP-A (VAPA Human)
UseTagsT7-Xpress tag and mCherryExpressionMammalianMutationK94D/M96D mutant, unable to bind FFAT motifsPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherryC1 VAP-B
Plasmid#226409PurposeExpression of human VAP-B fused to mCherry in mammalian cellsDepositorInsertVAP-B (VAPB Human)
UseTagsHA, T7-Xpress tag, and mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only