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Showing: 1241 - 1260 of 6476 results

  1. pET MBP mClover LIC cloning vector (MBP-mClover)

    Plasmid
    #86930
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    Biotin, His6, MBP, Tev Recognition Sequence, and …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86930-plasmid-map-sequence-id-174752

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  2. pFB-CT10HF-LIC

    Plasmid
    #39191
    Purpose
    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    TEV-His-FLAG
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39191-plasmid-map-sequence-id-136169

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  3. pET MBP tdTomato LIC cloning vector (MBP-tdTomato)

    Plasmid
    #86927
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-tdTomato
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    Biotin, His6, MBP, Tev Recognition Sequence, and …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86927-plasmid-map-sequence-id-181702

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  4. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Insert
    MSP1D1Q1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97326-plasmid-map-sequence-id-182622

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  5. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137643-plasmid-map-sequence-id-381376

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  6. 371-C

    Plasmid
    #97029
    Purpose
    His6-MBP-N10-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag, MBP domain, N10 linker, and TEV cleavag…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97029-plasmid-map-sequence-id-182500

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  7. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Use
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160929-plasmid-map-sequence-id-319094

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  8. pJK573

    Plasmid
    #72456
    Purpose
    Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~57 silent substitutions
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72456-plasmid-map-sequence-id-133910

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  12. GB1-PRD(703-868, P801G)-Strep

    Plasmid
    #164444
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
    Use
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164444-plasmid-map-sequence-id-324031

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  13. pOCC240

    Plasmid
    #118895
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and mCherry, clea…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118895-plasmid-map-sequence-id-232366

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  14. p5A0-T

    Plasmid
    #92016
    Purpose
    Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    J23102 Promoter (from Anderson promoter library) …
    Available since
    Jan. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    92016-plasmid-map-sequence-id-201851

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  15. pOCC177

    Plasmid
    #118886
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease and SNAP tag…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118886-plasmid-map-sequence-id-232379

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  16. pOCC210

    Plasmid
    #118896
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and SNAP tag, cle…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118896-plasmid-map-sequence-id-232311

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  18. pKG123

    Plasmid
    #63074
    Purpose
    Retroviral expression of mCherry-TEV-S-tag CENP-C for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-C (CENPC Human)
    Use
    Retroviral
    Tags
    TEV-S and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    63074-plasmid-map-sequence-id-135907

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  19. pKG187

    Plasmid
    #63077
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FAAP24, IMAGE clone 3659284 (FAAP24 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    63077-plasmid-map-sequence-id-134680

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  20. pKG186

    Plasmid
    #63076
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FANC-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FANC-A, IMAGE clone 5725803 (FANCA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    63076-plasmid-map-sequence-id-104390

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Showing: 1241 - 1260 of 6476 results