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Plasmid#127264PurposeFor in vitro translation of human ELL2 with Met133ILeu, Met186ILeuDepositorInsertELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationTwo Mets (133,186 )to ILeu QuikchangePromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met331Leu ELL2
Plasmid#127262PurposeFor in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met331 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet331 to ILeu QuikchangePromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met1ILeu ELL2
Plasmid#127258PurposeFor in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met1 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet1 to Ileu by QuikchangePromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-Cx43-FS
Plasmid#65440PurposeCloning, T7 RNA expressionDepositorInsertCx43 (GJA1 Human)
UseT7 in vitro rna productionTagsExpressionBacterialMutationFrameshift (FS) mutation 35delAPromoterT7Available sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PCP-T5Exo
Plasmid#214736Purposeencodes T5 exonuclease with a PP7 coat protein fused to the N terminusDepositorInsertCAG_PCP-T5Exo_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp
Plasmid#101349Purpose2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta tat delta TAR delta env GFP in Nef Tet ope…PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5188
Plasmid#120087PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.DepositorInsertpAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMW35 p21 (mouse)
Plasmid#16478DepositorInsertp21 (Cdkn1a Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pWAP-HGF
Plasmid#83503PurposeGeneration of transgenic mice overexpressing the HGF under the control of the WAP gene promoterDepositorInsertHGF (Hgf Mouse)
UseMouse TargetingTagsExpressionMutationalso contains beta-globin sequences from pUC198Promotermouse WAP (whey acidic protein)Available sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
Plasmid#158962PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed DsRed cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4
Plasmid#177265PurposeSTREAMING-tag cassetteDepositorInsertSTREAMING-tag cassette
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)
Plasmid#36880DepositorInsertstTA2
tdT-3Myc
insulator
UseTags3 Myc tagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTN4
Plasmid#174212PurposeDonor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locusDepositorInsertsUseCRISPR and Cre/LoxTagsmRubyExpressionWormMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHes7-Achilles-Hes7
Plasmid#153528PurposeExpresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.DepositorInsertHes7 (Hes7 Mouse)
UseTagsAchillesExpressionMammalianMutationPromotermouse Hes7 promoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
WWP-Luc (p21/WAF1 promoter)
Plasmid#16451DepositorInsertWAF1 promoter (CDKN1A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-GFPluc2
Plasmid#80493Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expressionDepositorInsertGFPluc2
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTBNde(min)
Plasmid#15125DepositorInsertalpha-globin EDB fibronectin minigene (FN1 Human)
UseTagsExpressionMammalianMutationPromoteralpha-globinAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1
Plasmid#184397PurposeDonor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1DepositorInsertFirefly luciferase beta-globin-PTC39
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPTC in beta-globin at amino acid position 39PromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lox-stop-Lox p53 R172H
Plasmid#14854DepositorInsertp53 R172H (Trp53 Mouse)
UseCre/LoxTagsLox-STOP-loxExpressionMammalianMutationStructural mutant R172H. (Murine p53 codon 172 co…PromoterAvailable sinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1
Plasmid#184398PurposeDonor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1DepositorInsertRenilla luciferase beta-globin-PTC39
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPTC in beta-globin at amino acid position 39PromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only