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Plasmid#186274PurposeExpresses Thiomicrospira Cas6-RT-Cas1 in E. coliDepositorInsertThiomicropira Cas6-RT-Cas1
UseTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA1031
Plasmid#189584PurposeEdH4gp0214 deletion HR vector 250bp HR Arms (EdH4 phage editing)DepositorInsertEdH4gp0214 Deletion Locus 250bp Homology Arms
UseSynthetic BiologyTagsExpressionBacterialMutationEncodes for a full deletion of EdH4gp0214PromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA1030
Plasmid#189582PurposeEdH4gp0004 deletion HR vector 250bp HR Arms (EdH4 phage editing)DepositorInsertEdH4gp0004 Deletion Locus 250bp Homology Arms
UseSynthetic BiologyTagsExpressionBacterialMutationEncodes for a full deletion of EdH4gp0004PromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH026_proD_Cas12c-multiplexing_CAM
Plasmid#183073PurposePlasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repressionDepositorInsertExpressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_K310A
Plasmid#91856PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) K310A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationK310APromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_D1078A
Plasmid#91860PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) D1078A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationD1078APromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_N314A
Plasmid#91858PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) N314A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationN314APromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_K1080A
Plasmid#91862PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) K1080A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationK1080APromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA6
Plasmid#83869PurposeGateway donor vector for IAA6DepositorInsertIAA6 (IAA6 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA7_no stop
Plasmid#83872PurposeGateway donor vector for IAA7 with no stop codonDepositorInsertIAA7 (IAA7 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA3
Plasmid#83862PurposeGateway donor vector for IAA3DepositorInsertIAA3 (SHY2 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA20_no stop
Plasmid#83859PurposeGateway donor vector for IAA20 with no stop codonDepositorInsertIAA20 (IAA20 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA19_no stop
Plasmid#83857PurposeGateway donor vector for IAA19 with no stop codonDepositorInsertIAA19 (IAA19 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA5
Plasmid#83867PurposeGateway donor vector for IAA5DepositorInsertNP_173011.1 (IAA5 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA31_no stop
Plasmid#83865PurposeGateway donor vector for IAA31 with no stop codonDepositorInsertNP_188387.1 (IAA31 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA2_no stop
Plasmid#83858PurposeGateway donor vector for IAA2 with no stop codonDepositorInsertIAA2 (IAA2 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA19
Plasmid#83856PurposeGateway donor vector for IAA19DepositorInsertIAA19 (IAA19 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA14_no stop
Plasmid#83852PurposeGateway donor vector for IAA14DepositorInsertIAA14 (IAA14 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA14
Plasmid#83851PurposeGateway donor vector for IAA14DepositorInsertIAA14 (IAA14 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only