Showing: 1241 - 1260 of 15987 results
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Plasmid#215012PurposeExpress a ERphagy reporter in mammalian cells (untested)DepositorInsertKDEL
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)
Plasmid#156404PurposeGateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codonDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseGateway cloningTagsmTagBFP2ExpressionMutationmutant KRAS4A-G12DPromoterAvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized to remove terminal 7 aa (QEEQEEY) (JB162))
Plasmid#39308DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationC-terminal 7 aa (QEEQEEY) removedPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)
Plasmid#117168PurposeCRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)
Plasmid#215002PurposeShuttling vector for an ERphagy reporterDepositorInsertKDEL
UseGatewayTagsHaloTag(7)-mGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
Plasmid#217970PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.DepositorInsert2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK
Plasmid#141452PurposeB6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy numberDepositorInsertCdk6
UseMouse Targeting; Dre / roxTagsC-terminal fusion of T2A-TagRFPT-dre-PBD*ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE
Plasmid#137994PurposeBackbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcodingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS
Plasmid#163083PurposeDonor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENsDepositorInsertTagRFP-T
UseTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf
Plasmid#175524PurposeCo-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cellsDepositorInsertBeta4Gal-HaloTag7 and Lyn11-SNAPf
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc
Plasmid#25458DepositorInsertPAM (Pam Rat)
UseTagsHis6 and PAM signalExpressionMammalianMutationContains aa 498-820 (PALcc= PAL catalytic core) …PromoterAvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k
Plasmid#127401PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
UseTagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k
Plasmid#127403PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
UseTagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k
Plasmid#127407PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
UseTagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon, K305H,…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
Plasmid#159874PurposeThree-fragment Gateway compatible protein tag for expression in C. elegansDepositorInsert[1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
Plasmid#159844PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag
Plasmid#186708PurposeN-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.DepositorInsertalr4766
UseSynthetic BiologyTagsHis-Tag (6His)ExpressionMutationPromoterPpsbA2*AvailabilityAcademic Institutions and Nonprofits only -
pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag
Plasmid#186710PurposeN-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.DepositorInsertalr4766
UseSynthetic BiologyTagsHis-Tag (6His)ExpressionMutationPromoterPtrc.x.tetO2AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag
Plasmid#187356PurposeBacterial expression and refolding of gp100 pMHCDepositorInserthHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
HOXB4 (human) HIS-tag pET new RBS
Plasmid#8525DepositorInsertHOXB4 (HOXB4 Human)
UseTagsHis and T7ExpressionBacterialMutationContains improved Ribosomal binding site; makes …PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1241 - 1260 of 15987 results