Addgene

We narrowed to 35,220 results for: lat

Showing: 1241 - 1260 of 35220 results

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pHR-mCherry-P2A-strepII-PPPCB

Plasmid

#67923

Purpose

Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pQE60 MGH

Plasmid

#204244

Purpose

Bacterial expression of a fusion of watermelon glyoxysomal malate dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MGH

Use

Tags

6X His and GFP

Expression

Bacterial

Mutation

fusion of MDH and GFP with His tag

Promoter

Lac

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Gal-Rad51

Plasmid

#33230

Depositor

Hannah Klein

Article

Shah et al Mol Cell. 2010 Sep 24;39(6):862-72.

Insert

Rad51 (RAD51 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Gal1

Available since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pMJB1

Plasmid

#160429

Purpose

Yeast vector for expressing mCherry tagged genes under the GAL1 promoter

Depositor

Gary Stormo

Article

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Type

Empty backbone

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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RSV CREB

Plasmid

#22394

Depositor

Marc Montminy

Article

Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

Insert

CREB (Creb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
I-2/pCS2MT

Plasmid

#16317

Depositor

Peter Klein

Article

Zhang et al J Biol Chem. 2003 Aug 29. 278(35):33067-

Insert

Inhibitor-2 (Ppp1r2 Mouse)

Use

Vertebrate expression

Tags

5x myc

Expression

Mutation

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pT2KXIGdeltaIn-MCS-birA-tagRFP

Plasmid

#58378

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA tagRFP

Use

Tags

tagRFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pIA1405

Plasmid

#172404

Purpose

Expression of SARS-COV-2 Nsp12 D218A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

D218A

Promoter

T7

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1420

Plasmid

#172406

Purpose

Expression of SARS-COV-2 Nsp12 R116A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

R116A

Promoter

T7

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1421

Plasmid

#172407

Purpose

Expression of SARS-COV-2 Nsp12 K50A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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NHA-QN

Plasmid

#36441

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A (TNRC6A Human)

Use

Tags

Expression

Mammalian

Mutation

aa 1194-1580 only

Promoter

Available since

Oct. 3, 2012

Availability

Academic Institutions and Nonprofits only
pT2KXIGdeltaIn-MCS-birA-EGFP

Plasmid

#58377

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA EGFP

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pSicoR-mCh-GFPi

Plasmid

#21905

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Jul 8. ():.

Insert

GFPi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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NHA-TNR

Plasmid

#36923

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNGW1 (TNRC6A Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

aa 1-204 only

Promoter

Available since

May 30, 2012

Availability

Academic Institutions and Nonprofits only
pIA1402

Plasmid

#172403

Purpose

Expression of SARS-COV-2 Nsp12 Y129A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Y129A

Promoter

T7

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-17-6

Plasmid

#190827

Purpose

For mammalian expression of 17-6, a non-aptamer RNA. Expression is driven by a U6 promoter.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

17-6 (Non-aptamer RNA)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-mitfa

Plasmid

#81230

Purpose

Cloning of zebrafish mitfa gene

Depositor

James Lister, David Raible

Article

Lister et al Development. 1999 Sep;126(17):3757-67.

Insert

mitfa (mitfa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T3

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pIA1408

Plasmid

#172405

Purpose

Expression of SARS-COV-2 Nsp12 D760A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

D760A

Promoter

T7

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCTX-myc-HIPK2 KD

Plasmid

#32997

Depositor

Sergei Sokol

Article

Hikasa et al Dev Cell. 2010 Oct 19;19(4):521-32.

Insert

HIPK2 (hipk2 Frog)

Use

Tags

6XMyc

Expression

Mutation

K217>R (in the catalytic lysine) and STY348-35…

Promoter

SCMV

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pAG414GPD-VuCYP722C(pYL1070)

Plasmid

#178293

Purpose

Expresses VuCYP722C from V. unguiculata in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VuCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

VuCYP722C

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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