Addgene

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pLNC Int3HA

Plasmid

#21329

Depositor

Jan Kitajewski

Article

Uyttendaele et al Dev Biol. 1998 Apr 15. 196(2):204-17.

Insert

Int3 (Notch4 Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
pOX + Shaker1c

Plasmid

#16140

Depositor

Larry Salkoff

Article

Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

Insert

Voltage-gated K+ channels (ZK1321.2 Nematode)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pOX + Shaker1d

Plasmid

#16141

Depositor

Larry Salkoff

Article

Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

Insert

Voltage-gated K+ channels (ZK1321.2 Nematode)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pUPD2+pEstrInd

Plasmid

#170880

Purpose

Phytobrick - Estradiol Inducible Promoter

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pEstradiol_Inducible

Use

Tags

Expression

Plant

Mutation

c.837C>T and c.1353G>A to remove BsaI and E…

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp2

Plasmid

#192782

Purpose

green biosensor for cAMP in mammalian cells

Depositor

Jun Chu

Article

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2

Use

Tags

His tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET-NT*-HRV3CP

Plasmid

#162795

Purpose

Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

Depositor

Gottfried Otting

Article

Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

Insert

Human rhinovirus 14 3C protease

Use

Tags

NT* solubility tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDU1

Plasmid

#169817

Purpose

pSpCas9(BB)-2A-tCD19 (pDU1)

Depositor

Andrea Rossi

Article

Ramachandran et al Life Sci Alliance. 2021 Apr 26;4(6). pii

Insert

tCD19

Use

Tags

fusion protein

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp2-mut

Plasmid

#192783

Purpose

control probe of G-Flamp2, insensitive to cAMP

Depositor

Jun Chu

Article

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2-mut

Use

Tags

His tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP

Plasmid

#162823

Purpose

Doxycycline-inducible overexpression of eGFP

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V163A

Promoter

Tet ON

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pG-HIV-2LTR

Plasmid

#104590

Purpose

standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of HIV-1

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pG-HIV-LRT

Plasmid

#104592

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

HIV-1 late reverse transcripts

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRU41

Plasmid

#167663

Purpose

ENTRY vector for gRNA1 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU42

Plasmid

#167664

Purpose

ENTRY vector for gRNA2 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-RhoBAST

Plasmid

#196341

Purpose

Overexpression of circular RhoBAST

Depositor

Murat Sunbul

Article

Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

Insert

Tornado-RhoBAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJG103

Plasmid

#158533

Purpose

wrmScarlet11 x3 tandem repeats

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet11 x3 tandem repeats

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-PhoCl2f-mCherry

Plasmid

#164048

Purpose

Optogenetic control of protein localization by PhoCl2f

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2f-mCherry

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pNB768

Plasmid

#60139

Purpose

Green click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Use

Tags

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-ChETA-EYFP

Plasmid

#26967

Purpose

3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Insert

hChR2(E123T/H134R)-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

E123T

Promoter

CaMKIIa

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pcNDA-NES-PhoCl2c-mCherry

Plasmid

#164037

Purpose

Optogenetic control of protein localization by PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2c-mCherry

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRU44

Plasmid

#167666

Purpose

ENTRY vector for gRNA4 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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