Addgene

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pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox

Plasmid

#196889

Purpose

Dre-activated Cre-inactivated expression of lyn-mScarlet reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

lyn-mScarlet

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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2M-Mx-FLAG

Plasmid

#206942

Purpose

Mammalian Expression of 2M-Mx-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, MxEnc

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBactin-Halo-Tag

Plasmid

#196898

Purpose

Expression of Halo-Tag. Use as control for Halo-tag fusions

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Halo-Tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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1M-Mx-FLAG

Plasmid

#206941

Purpose

Mammalian Expression of 1M-Mx-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, MxEnc

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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1M-Qt-FLAG

Plasmid

#206938

Purpose

Mammalian Expression of 1M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-CI

Plasmid

#196849

Purpose

Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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1M-Tm-BC2

Plasmid

#206943

Purpose

Mamamlian Expression of 1M-Tm-BC2Tag EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, TmEnc

Use

Tags

BC2

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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3M-Qt-FLAG

Plasmid

#206940

Purpose

Mammalian Expression of 3M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, SeSmtA, TaEC1, QtEnc

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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2M-Qt-FLAG

Plasmid

#206939

Purpose

Mammalian Expression of 2M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hPU.1ΔN165-pET28b

Plasmid

#199796

Purpose

Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

Depositor

Gregory Poon

Article

Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

Insert

spi1 (SPI1 Human)

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

Plasmid

#196877

Purpose

Neuron-specific expression of mOrange2 reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mOrange2

Plasmid

#196860

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mOrange2 (Nedd1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBactin-td-mCherry-CI

Plasmid

#196851

Purpose

Expression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

td-mCherry

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBactin-Tubg1-mEGFP

Plasmid

#196870

Purpose

Expression of the gamma-tubulin fused to enhanced (E)-GFP

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Tubg1-mEGFP (Tubg1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

Plasmid

#196874

Purpose

Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynGFP

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP

Plasmid

#196876

Purpose

Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynEGFP

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196883

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotrasfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

Plasmid

#196884

Purpose

Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotrasfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-Lyn-GFP-bGH

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196886

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotrasfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-lox-roxSTOProx-tRFP-pA-lox

Plasmid

#196888

Purpose

Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

turbo (t)RFP

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge