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pDEST51-LRRK2-Y1699C
Plasmid#25084DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationTyrosine 1699 mutated to CysteinePromoterAvailable sinceJuly 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
TLS 4: hTLS-CHOP.pBSKS(+)
Plasmid#21830DepositorInsertTLS-CHOP (FUS Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-MYC
Plasmid#18824DepositorInsertTRPML1 (MCOLN1 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
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mTagRFP-T-Paxillin-22
Plasmid#58024PurposeLocalization: Focal Adhesions, Excitation: 555, Emission: 584DepositorInsertPaxillin
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBabe-Puro-RalA-wt
Plasmid#15251DepositorInsertv-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP reporterExpressionYeastMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP-CLN2PEST reporterExpressionYeastMutationPromoterAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-PAmCherry1_160-236-MCS
Plasmid#60546PurposeCloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALMDepositorInsertPAmCherry1 C-terminal fragment
UseCloningTagsExpressionMutationamino acids 160-236PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-PAmCherry1_1-159-MCS
Plasmid#60545PurposeCloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALMDepositorInsertPAmCherry1 N-terminal fragment
UseCloningTagsExpressionMutationamino acids 1-159PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME-puro-CLIP-FLAG-CD59
Plasmid#50375PurposeExpress CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cellsDepositorInsertCLIP
UseTagsExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM55351
Plasmid#169092PurposeMoClo level 1 35S promoter-BvADHα-Nos terminator transcription unitDepositorInsert35S promoter-BvADHα-Nos terminator
UseMoclo cloning vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS mPrx1 Cre (CT#644)
Plasmid#13962DepositorInsertPrx1 Cre (Prrx1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)
Plasmid#70198PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-STf
Plasmid#182008PurposeCMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell linesDepositorInsertLyn11-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP
Plasmid#185686PurposeLow-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.DepositorInsertERmembrane-GST-HaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pISI-mmP16 3' UTR
Plasmid#22693DepositorInsertmmP16 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 RagD shRNA #2
Plasmid#30322DepositorInsertRagD shRNA (RRAGD Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
Kohinoor-Paxillin/pcDNA3
Plasmid#67775PurposePaxilin-targetted Kohinoor for mammalian cell expressionDepositorInsertKohinoor
UseTagsPaxillinExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Smad3 LC
Plasmid#12633DepositorInsertSmad3 LC (SMAD3 Human)
UseTagsGSTExpressionBacterialMutationLC segment of Smad3 (aa 146-425)PromoterAvailable sinceOct. 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S100C
Plasmid#190010PurposeExpression of human TTR in E coliDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S100CPromotert7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-eBFP2
Plasmid#67030PurposePlasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
UseConjugative bacterial vectorTagsExpressionMutationPromoterAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRPML3-CFP
Plasmid#18834DepositorInsertTRPML3 (Mcoln3 Mouse)
UseTagsCFPExpressionMammalianMutationQ165R, F210YPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1 mNFATc2
Plasmid#17858DepositorInsertNFATc2 (Nfatc2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pMINTC3GH (Apr)
Plasmid#110078PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRetroSuper-GFP TIF1gamma
Plasmid#15721DepositorInsertTIF1 gamma shRNA (TRIM33 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-RapR-p38
Plasmid#25935DepositorInsertRapR-p38 (Mapk14 Human, Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEE390
Plasmid#149280PurposeT-DNA encoding TRV2 with gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179077PurposeVector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosolDepositorInsertTDH3p-Neon(gfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNCX Myr His p110 KD
Plasmid#10837DepositorInsertp110 KD (PIK3CA Bovine)
UseRetroviralTags6xHis and MyrExpressionMammalianMutationD915S. Kinase Dead.PromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
Dppa1-T2A-H2B-Halo donor
Plasmid#113118PurposeDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequenceDepositorInsertDppa1 donor region including homology arms and T2A-H2B-Halo
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Kohinoor-Tublin/pcDNA3
Plasmid#67780PurposeTublin-targetted Kohinoor for mammalian cell expressionDepositorInsertKohinoor
UseTagsb-TubulinExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-HBH
Plasmid#26886DepositorInsertHBH tag N-terminal
UsePcr tagging yeastTagsExpressionMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pARS3_MUbCAS9_GW
Plasmid#128646PurposeT-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNADepositorTypeEmpty backboneUseTagsHAExpressionPlantMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Apr)
Plasmid#110083PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
cr3Dd
Plasmid#184862Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination systemDepositorInsertCas9, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5 dOrai
Plasmid#16368DepositorInsertOrai (olf186-F Fly)
UseTagsV5ExpressionInsectMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-mMcl-1
Plasmid#32984DepositorInsertMcl-1 (Mcl1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJH579
Plasmid#172163PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD JM10.1); AraC
UseTagsExpressionBacterialMutationSumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shRalA-2
Plasmid#15258DepositorInsertv-ral simian leukemia viral oncogene homolog A (RALA Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)
Plasmid#178996PurposeSp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Control-miR-17-5p-Control
Plasmid#21167DepositorInsertmiR 17-5p Sensor (MIR17 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
EZ-L767
Plasmid#131773PurposePADH1_sFUS_Fusion Red_PixD_TACT1DepositorInsertPADH1_sFUS_Fusion Red_PixD_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x5
Plasmid#58775PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-RalA-V23
Plasmid#15252DepositorInsertv-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationchanged 23rd amino acid to valinePromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)
Plasmid#178995PurposeSp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Smad3 C
Plasmid#12634DepositorInsertSmad3 C (SMAD3 Human)
UseTagsGSTExpressionBacterialMutationC segment of Smad3 (aa 199-425)PromoterAvailable sinceOct. 10, 2006AvailabilityAcademic Institutions and Nonprofits only
