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Plasmid#52258PurposeBacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only
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pCH67 (crRNA expression vector)
Plasmid#217337PurposeCROP-seq crRNA expression vector with GFP and puro markerDepositorInsertAsDR-filler-AsDR8, GFP-puro
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Tet1
Plasmid#60938PurposeExpresses Tet1 in mammalian cellsDepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1
Plasmid#58437PurposeExpresses LexA-ER-B112 in yeast cellsDepositorInsertLexA-ER-B112
UseTagsExpressionYeastMutationPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
nls-mCherry
Plasmid#108881PurposeExpression of nuclear localized mCherryDepositorInsertnls-mCherry
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorInsertcAMPFIRE-L (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)
Plasmid#128165PurposeUV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + 5alanines
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialMutationPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC101-AmpR EV
Plasmid#208184Purposeempty plasmid vector, non-fluorescentDepositorTypeEmpty backboneUseReporterTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1
Plasmid#213025PurposeExpresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cellsDepositorInsertLINE-1 ORF2p (1-1275)
UseTags3C-3xFlag (ORF2p)ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase
Plasmid#87741PurposeIPTG-inducible expression of T4 DNA ligase for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHisExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only