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pFH2.2_mCherry-MS2x12

Plasmid

#205521

Purpose

Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEF6-Acly(S455D)

Plasmid

#70768

Purpose

Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Use

Tags

myc-epitope

Expression

Mammalian

Mutation

S455D

Promoter

EF1alpha

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Express a lysosomal Tag

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Expression

Mutation

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HDAC2

Plasmid

#222553

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HDAC2 (HDAC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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2C::tdTomato Reporter

Plasmid

#40281

Depositor

Samuel Pfaff

Article

Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

Insert

2C Promoter (2-cell stage promoter)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

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plenti-CAG -IRES-GFP

Plasmid

#69047

Purpose

Lentiviral backbone for GFP coexpression

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDKN1A_WT

Plasmid

#82201

Purpose

Gateway Donor vector containing CDKN1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDKN1A (CDKN1A Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-sL7-Cre-HA-WPRE

Plasmid

#204488

Purpose

For production of driver AAV virus for Purkinje cell-specific expression

Depositor

Samuel Wang

Article

Chen et al Nature. 2022 May;605(7911):722-727. doi:

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

sL7

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 WT

Plasmid

#199573

Purpose

Expresses FLAG-tagged WT SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDule-para-aminoPhe

Plasmid

#85502

Purpose

Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

Insert

p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Use

Tags

None

Expression

Bacterial

Mutation

Y32T, E107T, D158P, I159L, L162A

Promoter

lpp (constitutive)

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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