Addgene

We narrowed to 1,666 results for: rpe

Showing: 1221 - 1240 of 1666 results

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pLJC5-Flag-SAMTOR

Plasmid

#102420

Purpose

3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoter

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTACR TH1-p2aGFP

Plasmid

#135815

Purpose

CRISPR based on px330 p2aGFP targeting TH gene near stop codon

Depositor

Lee Rubin, Tim Ahfeldt

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH 1 guide

Use

CRISPR

Tags

p2a Myr-eGFP

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTACR TH2-p2aGFP

Plasmid

#135816

Purpose

CRISPR based on px330 p2aGFP targeting TH gene near stop codon

Depositor

Lee Rubin, Tim Ahfeldt

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH 2 guide

Use

CRISPR

Tags

p2a Myr-eGFP

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-CYLD-STOP

Plasmid

#60077

Purpose

N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

Plasmid

#167268

Purpose

Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HOB

Use

Tags

His x10

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-FLAG-CYLD-WT

Plasmid

#60030

Purpose

WT CYLD under control of Tet promoter with N-terminal FLAG tag

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only
pEPQD0KN0750

Plasmid

#185627

Purpose

Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

lacZ

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ZP60 Axin2

Plasmid

#16882

Depositor

Randall Moon

Article

Weidinger et al Curr Biol. 2005 Mar 29. 15(6):489-500.

Insert

axin2 (axin2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pLC242-GFP-metap2

Plasmid

#100518

Purpose

Expresses GFP tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-CLIP-tag_fast

Plasmid

#167275

Purpose

Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIPf-tag

Use

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR2-Flag Cterm

Plasmid

#84490

Purpose

overexpression of CASTOR2 with Flag tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 (GATSL2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
M77 pIRESpuro-GLUE-hDsh3

Plasmid

#15102

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

Dsh3 (DVL3 Human)

Use

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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M76 pIRESpuro-GLUE-hDsh2

Plasmid

#15101

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

Dsh2 (DVL2 Human)

Use

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CG2APtk

Plasmid

#86003

Purpose

homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

eGFP_T2A_puro_dTK

Use

Piggybac exciseable

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-CYLD-C601A

Plasmid

#60028

Purpose

N-terminal GFP tagged catalytic inactive (C601A) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Cysteine 601 changed to Alanine

Promoter

CMV

Available since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

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p6346 MSCV-CMV-Flag-HA-Brd4-1-444

Plasmid

#32886

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 (BRD4 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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p7053 pHAGE-P-CMVt-N-HA-GAW-BICD2

Plasmid

#100143

Purpose

Expresses BICD2

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

BICD2 (BICD2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-GLI(-)TAD

Plasmid

#37113

Depositor

David Walterhouse

Article

Yoon et al J Biol Chem. 1998 Feb 6;273(6):3496-501.

Insert

GLI1 (GLI1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted 1,409 bp AccI fragment from 3' end o…

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
p7127 pHAGE-P-CMVt-N-HA-GAW-FTL

Plasmid

#100153

Purpose

Expresses FTL

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

FTL (FTL Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1-2xStrep

Plasmid

#138436

Purpose

Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Use

Tags

Strep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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